肝硬化腹水患者的腹膜巨噬细胞表现出吞噬功能受损和强烈的呼吸爆发。
Peritoneal macrophages from patients with cirrhotic ascites show impaired phagocytosis and vigorous respiratory burst.
作者信息
Ahmed Abdel Motaal M, Bomford Adrian, Nouri-Aria Kayhan T, Davies Ted, Smith Roger, Williams Roger
机构信息
Institute of Liver Studies, King's Denmark Hill Campus, Bessemer Road, London SE5 9PJ, UK.
The Institute of Hepatology, The Foundation for Liver Research, 69-75 Chenies Mews, London WC1E 6HX, UK.
出版信息
Results Immunol. 2011 Sep 1;1(1):53-9. doi: 10.1016/j.rinim.2011.08.004. eCollection 2011.
Cirrhotic patients (CPs) are susceptible to spontaneous bacterial peritonitis (SBP). Aim of this study was to examine if this susceptibility was related to peritoneal macrophages' (PMs) altered host defence. Absorbance of phagocytosed particles by PMs from CPs was lower than that of control (31.88% vs. 77.2%). Particle opsonisation increased the absorbance to 41% in CPs' PMs, and this value remains lower than the control; 77.2%. Respiratory burst (RB) was expressed as fluorescence index values, and these were higher in PMs from CPs than in controls (82 vs. 41, 73 vs. 26 and 71 vs. 26). IFN-γ made no further increase of RB values in PMs from CPs. CD14 expression was also higher in CPs' PMs. IFN-γ significantly downregulated CD14 expression in both CPs' PMs and control. Reduced phagocytosis by predominantly CD14-positive PMs from CPs could be related to intense RB. Findings suggest altered host defence that could contribute to susceptibility to SBP.
肝硬化患者（CPs）易患自发性细菌性腹膜炎（SBP）。本研究的目的是检验这种易感性是否与腹膜巨噬细胞（PMs）宿主防御功能改变有关。CPs的PMs对吞噬颗粒的吸光度低于对照组（31.88%对77.2%）。颗粒调理作用使CPs的PMs吸光度增加至41%，但该值仍低于对照组；77.2%。呼吸爆发（RB）以荧光指数值表示，CPs的PMs中的荧光指数值高于对照组（82对41、73对26和71对26）。IFN-γ未使CPs的PMs中的RB值进一步增加。CPs的PMs中CD14表达也较高。IFN-γ显著下调了CPs的PMs和对照组中的CD14表达。CPs中主要为CD14阳性的PMs吞噬作用降低可能与强烈的RB有关。研究结果表明宿主防御功能改变可能导致对SBP的易感性。
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