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Rapid non-radioactive TMACl hybridization protocol employing enzymatically labeled oligonucleotides.

作者信息

Sorg U, Enczmann J, Sorg R, Wernet P

机构信息

Immunology Laboratory, Heinrich-Heine University of Düsseldorf, FRG.

出版信息

Nucleic Acids Res. 1991 Sep 11;19(17):4782. doi: 10.1093/nar/19.17.4782.

DOI:10.1093/nar/19.17.4782
PMID:1891374
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC328736/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a56/328736/3860dca98384/nar00097-0193-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a56/328736/3860dca98384/nar00097-0193-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a56/328736/3860dca98384/nar00097-0193-a.jpg

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本文引用的文献

1
Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries.在氯化铵中与碱基组成无关的杂交：一种用于高度复杂基因文库寡核苷酸筛选的方法。
Proc Natl Acad Sci U S A. 1985 Mar;82(6):1585-8. doi: 10.1073/pnas.82.6.1585.
2
A method for biotinylating oligonucleotide probes for use in molecular hybridizations.一种用于分子杂交的寡核苷酸探针生物素化方法。
DNA. 1986 Aug;5(4):333-7. doi: 10.1089/dna.1986.5.333.
3
The thermal stability of oligonucleotide duplexes is sequence independent in tetraalkylammonium salt solutions: application to identifying recombinant DNA clones.
在四烷基铵盐溶液中，寡核苷酸双链体的热稳定性与序列无关：应用于鉴定重组DNA克隆。
Nucleic Acids Res. 1988 May 25;16(10):4637-50. doi: 10.1093/nar/16.10.4637.
4
Nonradioactive labeling of synthetic oligonucleotide probes with terminal deoxynucleotidyl transferase.
Anal Biochem. 1988 Mar;169(2):376-82. doi: 10.1016/0003-2697(88)90299-0.
5
T200 alternate exon use in murine lymphoid cells determined by reverse transcription-polymerase chain reaction.通过逆转录-聚合酶链反应确定小鼠淋巴细胞中T200可变外显子的使用情况。
J Immunol. 1989 Jul 1;143(1):315-21.