寡脱氧核糖核苷酸的化学和酶促生物素标记
Chemical and enzymatic biotin-labeling of oligodeoxyribonucleotides.
作者信息
Kempe T, Sundquist W I, Chow F, Hu S L
出版信息
Nucleic Acids Res. 1985 Jan 11;13(1):45-57. doi: 10.1093/nar/13.1.45.
Abstract
Biotin has been converted to 2-(biotinylamido)ethanol and condensed to phosphorylated oligonucleotides in a solid phase synthesis. The 5'-biotinylated oligonucleotides were enzymatically coupled to other DNA fragments by T4 DNA ligase or T4 RNA ligase. The hybridization properties of such biotin-labeled oligonucleotide probes were studied.
摘要
生物素已被转化为2-（生物素酰胺基）乙醇，并在固相合成中与磷酸化寡核苷酸缩合。5'-生物素化寡核苷酸通过T4 DNA连接酶或T4 RNA连接酶与其他DNA片段进行酶促偶联。研究了此类生物素标记寡核苷酸探针的杂交特性。
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