• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

Direct sequencing by thermal asymmetric PCR.

作者信息

Mazars G R, Moyret C, Jeanteur P, Theillet C G

机构信息

CNRS UA 1191, Val d'Aurelle-Centre Paul Lamarque, Montpellier, France.

出版信息

Nucleic Acids Res. 1991 Sep 11;19(17):4783. doi: 10.1093/nar/19.17.4783.

DOI:10.1093/nar/19.17.4783
PMID:1891375
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC328737/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcd9/328737/dfe69afc02f6/nar00097-0194-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcd9/328737/dfe69afc02f6/nar00097-0194-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcd9/328737/dfe69afc02f6/nar00097-0194-a.jpg

相似文献

1
Direct sequencing by thermal asymmetric PCR.
Nucleic Acids Res. 1991 Sep 11;19(17):4783. doi: 10.1093/nar/19.17.4783.
2
Rapid sequencing of unpurified PCR products by thermal asymmetric PCR cycle sequencing using unlabeled sequencing primers.使用未标记的测序引物通过热不对称PCR循环测序对未纯化的PCR产物进行快速测序。
Nucleic Acids Res. 1993 Jul 11;21(14):3333-4. doi: 10.1093/nar/21.14.3333.
3
Direct cycle sequencing of mutated alleles detected by PCR single-strand conformation polymorphism analysis.通过聚合酶链反应单链构象多态性分析检测到的突变等位基因的直接循环测序。
Biotechniques. 1993 May;14(5):790-4.
4
DNA-dependent protein kinase inhibitor (OK-1035) suppresses p21 expression in HCT116 cells containing wild-type p53 induced by adriamycin.DNA依赖性蛋白激酶抑制剂(OK-1035)可抑制阿霉素诱导的含野生型p53的HCT116细胞中p21的表达。
Biochem Biophys Res Commun. 1996 Apr 16;221(2):207-12. doi: 10.1006/bbrc.1996.0575.
5
Alteration of p53 and p21 during hepatocarcinogenesis in tree shrews.树鼩肝癌发生过程中p53和p21的变化
World J Gastroenterol. 2004 Dec 15;10(24):3559-63. doi: 10.3748/wjg.v10.i24.3559.
6
K-ras mutations and p53 alterations in neoplastic and nonneoplastic lesions associated with longstanding ulcerative colitis.与长期溃疡性结肠炎相关的肿瘤性和非肿瘤性病变中的K-ras突变和p53改变。
Am J Pathol. 1994 Apr;144(4):767-75.
7
Expression and significance of p53, rb, p21/waf-1, p16/ink-4a, and PTEN tumor suppressors in canine melanoma.p53、rb、p21/waf-1、p16/ink-4a和PTEN肿瘤抑制因子在犬黑色素瘤中的表达及意义
Vet Pathol. 2002 Jul;39(4):458-72. doi: 10.1354/vp.39-4-458.
8
Large-scale production of DNA sequencing templates by microtitre format PCR.通过微量滴定板形式的聚合酶链式反应大规模生产DNA测序模板。
Nucleic Acids Res. 1993 Jan 11;21(1):173-4. doi: 10.1093/nar/21.1.173.
9
Tumorigenicity of human induced pluripotent stem cells depends on the balance of gene expression between p21 and p53.
Hepatology. 2010 Mar;51(3):1088-9. doi: 10.1002/hep.23396.
10
Efficient production of single-stranded DNA as long as 2 kb for sequencing of PCR-amplified DNA.高效生产长达2 kb的单链DNA用于PCR扩增DNA的测序。
Biotechniques. 1992 Feb;12(2):164, 166, 168-71.

引用本文的文献

1
Protocol to Mine Unknown Flanking DNA Using PER-PCR for Genome Walking.利用PER-PCR进行基因组步移挖掘未知侧翼DNA的实验方案
Bio Protoc. 2025 Feb 20;15(4):e5188. doi: 10.21769/BioProtoc.5188.
2
Protocol to Identify Unknown Flanking DNA Using Partially Overlapping Primer-based PCR for Genome Walking.使用基于部分重叠引物的PCR进行基因组步移以鉴定未知侧翼DNA的方案
Bio Protoc. 2025 Feb 5;15(3):e5172. doi: 10.21769/BioProtoc.5172.
3
Efficient assembly of long DNA fragments and multiple genes with improved nickase-based cloning and Cre/loxP recombination.

本文引用的文献

1
Generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus.通过聚合酶链反应生成单链DNA及其在HLA - DQA基因座直接测序中的应用。
Proc Natl Acad Sci U S A. 1988 Oct;85(20):7652-6. doi: 10.1073/pnas.85.20.7652.
2
A variation in the structure of the protein-coding region of the human p53 gene.人类p53基因蛋白质编码区结构的一种变异。
Gene. 1988 Oct 30;70(2):245-52. doi: 10.1016/0378-1119(88)90196-5.
3
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.
高效组装长 DNA 片段和多个基因,采用改进的基于 Nickase 的克隆和 Cre/loxP 重组技术。
Plant Biotechnol J. 2022 Oct;20(10):1983-1995. doi: 10.1111/pbi.13882. Epub 2022 Jul 16.
4
A low degenerate primer pool improved the efficiency of high-efficiency thermal asymmetric interlaced PCR to amplify T-DNA flanking sequences in .一个低简并引物库提高了高效热不对称交错PCR在扩增……中T-DNA侧翼序列的效率。
3 Biotech. 2018 Jan;8(1):14. doi: 10.1007/s13205-017-1032-y. Epub 2017 Dec 11.
5
Partially overlapping primer-based PCR for genome walking.用于基因组步移的基于部分重叠引物的聚合酶链式反应
PLoS One. 2015 Mar 26;10(3):e0120139. doi: 10.1371/journal.pone.0120139. eCollection 2015.
6
Integrative gene transfer in the truffle Tuber borchii by Agrobacterium tumefaciens-mediated transformation.利用根癌农杆菌介导转化法实现块菌属 Tuber borchii 的基因整合。
AMB Express. 2014 May 29;4:43. doi: 10.1186/s13568-014-0043-x. eCollection 2014.
7
RNA editing events in mitochondrial genes by ultra-deep sequencing methods: a comparison of cytoplasmic male sterile, fertile and restored genotypes in cotton.基于超高深度测序方法的线粒体基因 RNA 编辑事件:棉花细胞质雄性不育、可育和恢复基因型的比较。
Mol Genet Genomics. 2013 Sep;288(9):445-57. doi: 10.1007/s00438-013-0764-6. Epub 2013 Jun 29.
8
Establishment of an improved high-efficiency thermal asymmetric interlaced PCR for identification of genomic integration sites mediated by phiC31 integrase.建立改良的高效热不对称交错 PCR 法鉴定 phiC31 整合酶介导的基因组整合位点。
World J Microbiol Biotechnol. 2012 Mar;28(3):1295-9. doi: 10.1007/s11274-011-0877-1. Epub 2011 Sep 10.
9
Inheritance of DNA transferred from American trypanosomes to human hosts.从美洲锥虫向人类宿主转移的 DNA 的遗传。
PLoS One. 2010 Feb 12;5(2):e9181. doi: 10.1371/journal.pone.0009181.
10
Multiplex asymmetric PCR-based oligonucleotide microarray for detection of drug resistance genes containing single mutations in Enterobacteriaceae.基于多重不对称PCR的寡核苷酸微阵列用于检测肠杆菌科中含有单突变的耐药基因。
Antimicrob Agents Chemother. 2007 Oct;51(10):3707-13. doi: 10.1128/AAC.01461-06. Epub 2007 Jul 23.
使用热稳定DNA聚合酶进行引物引导的DNA酶促扩增。
Science. 1988 Jan 29;239(4839):487-91. doi: 10.1126/science.2448875.
4
Optimization of asymmetric polymerase chain reaction for rapid fluorescent DNA sequencing.用于快速荧光DNA测序的不对称聚合酶链反应的优化
Biotechniques. 1990 Feb;8(2):184-9.