Amaral-Zettler Linda, Peplies Jörg, Ramette Alban, Fuchs Bernhard, Ludwig Wolfgang, Glöckner Frank Oliver
The Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, MA 02543, USA.
Syst Appl Microbiol. 2008 Sep;31(4):258-68. doi: 10.1016/j.syapm.2008.08.004.
Thirty years have passed since Carl Woese proposed three primary domains of life based on the phylogenetic analysis of ribosomal RNA (rRNA) genes. Adopted by researchers worldwide, rRNA has become the "gold-standard" for molecular taxonomy, biodiversity analysis and the identification of microorganisms. The more than 700,000 rRNA sequences in public databases constitute an unprecedented hallmark of the richness of microbial biodiversity on earth. The International Workshop on Ribosomal RNA Technology convened on April 7-9, 2008 in Bremen, Germany (http://www.arb-silva.de/rrna-workshop) to summarize the current status of the field and strategize on the best ways of proceeding on both biological and technological fronts. In five sessions, 26 leading international speakers and approximately 120 participants representing diverse disciplines discussed new technological approaches to address three basic ecological questions: "Who is out there?" "How many are there?" and "What are they doing?".
自卡尔·乌斯（Carl Woese）基于核糖体RNA（rRNA）基因的系统发育分析提出生命的三个主要域以来，已经过去了三十年。rRNA被全球研究人员采用，已成为分子分类学、生物多样性分析和微生物鉴定的“金标准”。公共数据库中的70多万条rRNA序列构成了地球上微生物生物多样性丰富程度的前所未有的标志。2008年4月7日至9日，核糖体RNA技术国际研讨会在德国不来梅召开（http://www.arb-silva.de/rrna-workshop），以总结该领域的现状，并就生物和技术前沿的最佳发展方式制定战略。在五场会议中，26位国际知名演讲者和来自不同学科的约120名与会者讨论了应对三个基本生态问题的新技术方法：“那里有谁？”“有多少？”以及“它们在做什么？”
