在域细菌内捕获更大的 16S rRNA 基因序列多样性。
Capturing greater 16S rRNA gene sequence diversity within the domain Bacteria.
机构信息
School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales, Australia.
出版信息
Appl Environ Microbiol. 2012 Aug;78(16):5938-41. doi: 10.1128/AEM.01299-12. Epub 2012 Jun 8.
Abstract
A large proportion of "universal" 16S PCR primers lack sequence homology to many of the "candidate" divisions, severely limiting bacterial diversity assessments. We designed a primer set that offers a 50% increase in silico in coverage of the domain Bacteria over the commonly used primer combination 27F/519R. Comparisons using pyrosequencing on soil environments showed a significant increase in recovery of taxonomic diversity with around a 3-fold increase in recovery of sequences from candidate divisions.
摘要
大量的“通用”16S PCR 引物缺乏与许多“候选”分类单元的序列同源性，严重限制了细菌多样性评估。我们设计了一套引物，与常用的引物组合 27F/519R 相比，在计算机模拟中对细菌域的覆盖率增加了 50%。使用焦磷酸测序对土壤环境进行比较的结果表明，候选分类单元的序列回收量增加了约 3 倍，分类多样性的回收量也显著增加。
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