乳酸乳球菌产生具有生物活性的人淋巴细胞趋化因子（XCL1）。

Production of biologically active human lymphotactin (XCL1) by Lactococcus lactis.

作者信息

Zavala-Flores Laura M, Villatoro-Hernandez Julio, Gamez-Escobedo Anali, Franco-Molina Moisés, Rangel-Colmenero Blanca R, Villanueva-Olivo Arnulfo, Gutierrez-Puente Yolanda, de Oca-Luna Roberto Montes, Valdés-Flores Jesús, Saucedo-Cardenas Odila

机构信息

Departamento de Histología, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey, NL, Mexico.

出版信息

Biotechnol Lett. 2009 Feb;31(2):215-20. doi: 10.1007/s10529-008-9855-6. Epub 2008 Oct 16.

DOI:10.1007/s10529-008-9855-6

PMID:18923910

Abstract

Lymphotactin-XCL1 is a chemokine produced mainly by activated CD8+ T-cells and directs migration of CD4+ and CD8+ lymphocytes and natural killer (NK) cells. We expressed human lymphotactin (LTN) by the lactic-acid bacterium Lactococcus lactis. Biological activity of LTN was confirmed by chemo-attraction of human T-cells by chemotaxis demonstrating, for the first time, how this chemokine secreted by a food-grade prokaryote retains biological activity and chemoattracts T lymphocytes. This strain thus represents a feasible well-tolerated vector to deliver active LTN at a mucosal level.

摘要

淋巴细胞趋化因子-XCL1是一种主要由活化的CD8+ T细胞产生的趋化因子，可引导CD4+和CD8+淋巴细胞以及自然杀伤（NK）细胞迁移。我们通过乳酸乳球菌表达了人淋巴细胞趋化因子（LTN）。通过趋化性对人T细胞进行化学吸引，证实了LTN的生物活性，首次证明了这种由食品级原核生物分泌的趋化因子如何保留生物活性并化学吸引T淋巴细胞。因此，该菌株是一种可行的、耐受性良好的载体，可在粘膜水平递送活性LTN。