Bochorishvili Ekaterine, Abramidze Tamar, Mgaloblishvili Nana, Shengelidze George, Gotua Maia
Department of Immunology, Center of Allergy and Immunology, Tbilisi, Georgia.
PhD student, David Tvildiani Medical University, Tbilisi, Georgia.
Postepy Dermatol Alergol. 2023 Aug;40(4):534-541. doi: 10.5114/ada.2023.130482. Epub 2023 Sep 1.
In allergy diagnostics, component-resolved diagnostics (CRD) allows the clinician to assess the presence of specific IgE (sIgE) to allergenic proteins. Molecular diagnostics has improved our ability to identify not only species-specific, but also panallergen components.
To characterize the Georgian allergic population according to the most frequently recognized plant panallergen components (profilins, PR-10 and nonspecific lipid transfer proteins) using sensitization data from multiplex CRD and investigate their association with particular allergic diseases.
Patients, IgE positive to at least one studied panallergen component, were selected out of total 435 allergic individuals and stratified in two age groups: children and adults. Descriptive statistics, Chi square test (χ) and Pearson Correlation test () were used for analysing the data.
38% (164/435) of investigated allergic patients showed IgE reactivity to at least one molecule belonging to Profilin, PR-10 and nsLTP families. Generally, PR-10 reactive individuals represented the largest group of patients (56.0%), followed by Profilins (43.0%) and nsLTP (32.0%). For the PR-10 allergen group, IgE sensitization was dominated by Bet v 1, for Profilin - by Hev b 8 and for nsLTP - by Pru p 3. It was shown that sensitization with nsLTPs revealed statistically important associations with allergic rhinitis ( = 0.005) and dermatitis ( = 0.02). PR-10 allergen sensitization was associated with allergic rhinitis ( = 0.04) and asthma ( = 0.04).
According to our results, sensitization to plant panallergens in the Georgian population creates characteristic features overlapping serotypes of Central Europe and Mediterranean region.
在过敏诊断中，组分分辨诊断（CRD）使临床医生能够评估针对变应原蛋白的特异性IgE（sIgE）的存在情况。分子诊断不仅提高了我们识别物种特异性变应原组分的能力，还提升了识别泛变应原组分的能力。
利用多重CRD的致敏数据，根据最常识别的植物泛变应原组分（肌动蛋白结合蛋白、PR-10和非特异性脂质转移蛋白）对格鲁吉亚过敏人群进行特征分析，并研究它们与特定过敏性疾病的关联。
从435名过敏个体中选出对至少一种研究的泛变应原组分IgE呈阳性的患者，并分为两个年龄组：儿童和成人。使用描述性统计、卡方检验（χ）和皮尔逊相关检验（）分析数据。
38%（164/435）的受调查过敏患者对属于肌动蛋白结合蛋白、PR-10和nsLTP家族的至少一种分子表现出IgE反应性。一般来说，对PR-10有反应的个体占患者的最大群体（56.0%），其次是肌动蛋白结合蛋白（43.0%）和nsLTP（32.0%）。对于PR-10变应原组，IgE致敏以Bet v 1为主，对于肌动蛋白结合蛋白以Hev b 8为主，对于nsLTP以Pru p 3为主。结果显示，nsLTP致敏与过敏性鼻炎（=0.005）和皮炎（=0.02）存在统计学上的显著关联。PR-10变应原致敏与过敏性鼻炎（=0.04）和哮喘（=0.04）相关。
根据我们的结果，格鲁吉亚人群对植物泛变应原的致敏呈现出与中欧和地中海地区血清型重叠的特征。
