Gabrilovich D I, Kozhich A T, Rosly I M, Suvorova Z K, Ivanov V S, Moshnikov S A, Tchikin L D, Melichov V I, Pokrovsky V V
Laboratory of Epidemiology and Prevention of AIDS, Central Institute of Epidemiology USSR Ministry of Public Health, Moscow.
AIDS. 1991 Jul;5(7):889-92. doi: 10.1097/00002030-199107000-00016.
The influence of HIV lysate and eight synthetic peptides which are fragments of HIV proteins on the functional activity of polymorphonuclear neutrophils (PMN) was tested in 12 healthy subjects. PMN activity in nitroblue tetrazolium reduction (NBT test) and PMN chemiluminescence (CL) was studied. Only one peptide was found to result in a significant increase in NBT test on the whole blood. This was the oligopeptide (G-97) from the CD4-binding site of HIV-1 gp120. The increase of CL response of PMN in the presence of G-97 was revealed after only 15 min preincubation. The same effect in the presence of sera from healthy or infected patients at the persistent generalized lymphadenopathy stage was achieved by increasing the time of preincubation to 30 min. G-97 did not influence the proliferative activity of lymphocytes.
在12名健康受试者中测试了HIV裂解物以及作为HIV蛋白片段的8种合成肽对多形核中性粒细胞（PMN）功能活性的影响。研究了PMN在硝基蓝四唑还原试验（NBT试验）中的活性以及PMN化学发光（CL）。仅发现一种肽可导致全血NBT试验显著增加。这是来自HIV-1 gp120 CD4结合位点的寡肽（G-97）。仅预孵育15分钟后，就发现G-97存在时PMN的CL反应增加。在持续性全身性淋巴结病阶段，通过将健康或感染患者血清存在时的预孵育时间延长至30分钟，可达到相同效果。G-97不影响淋巴细胞的增殖活性。
