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在高岭土诱导的交通性脑积水大鼠模型中,脑脊液流出阻力升高与淋巴系统脑脊液吸收受损有关。

Elevated CSF outflow resistance associated with impaired lymphatic CSF absorption in a rat model of kaolin-induced communicating hydrocephalus.

作者信息

Nagra Gurjit, Wagshul Mark E, Rashid Shams, Li Jie, McAllister J Pat, Johnston Miles

机构信息

Department of Laboratory Medicine and Pathobiology, Sunnybrook Health Sciences Centre, University of Toronto, Toronto, Ontario, Canada.

出版信息

Cerebrospinal Fluid Res. 2010 Feb 10;7(1):4. doi: 10.1186/1743-8454-7-4.

Abstract

BACKGROUND

We recently reported a lymphatic cerebrospinal fluid (CSF) absorption deficit in a kaolin model of communicating hydrocephalus in rats with ventricular expansion correlating negatively with the magnitude of the impediment to lymphatic function. However, it is possible that CSF drainage was not significantly altered if absorption at other sites compensated for the lymphatic defect. The purpose of this study was to investigate the impact of the lymphatic absorption deficit on global CSF absorption (CSF outflow resistance).

METHODS

Kaolin was injected into the basal cisterns of Sprague Dawley rats. The development of hydrocephalus was assessed using magnetic resonance imaging (MRI). In one group of animals at about 3 weeks after injection, the movement of intraventricularly injected iodinated human serum albumin (125I-HSA) into the olfactory turbinates provided an estimate of CSF transport through the cribriform plate into nasal lymphatics (n = 18). Control animals received saline in place of kaolin (n = 10). In a second group at about 3.5 weeks after kaolin injection, intraventricular pressure was measured continuously during infusion of saline into the spinal subarachnoid space at various flow rates (n = 9). CSF outflow resistance was calculated as the slope of the steady-state pressure versus flow rate. Control animals for this group either received no injections (intact: n = 11) or received saline in place of kaolin (n = 8).

RESULTS

Compared to saline injected controls, lateral ventricular volume in the kaolin group was significantly greater (0.087 +/- 0.013 ml, n = 27 versus 0.015 +/- 0.001 ml, n = 17) and lymphatic function was significantly less (2.14 +/- 0.72% injected/g, n = 18 versus 6.38 +/- 0.60% injected/g, n = 10). Additionally, the CSF outflow resistance was significantly greater in the kaolin group (0.46 +/- 0.04 cm H2O microL(-1) min, n = 9) than in saline injected (0.28 +/- 0.03 cm H2O microL(-1) min, n = 8) or intact animals (0.18 +/- 0.03 cm H2O microL(-1) min, n = 11). There was a significant positive correlation between CSF outflow resistance and ventricular volume.

CONCLUSIONS

The data suggest that the impediment to lymphatic CSF absorption in a kaolin-induced model of communicating hydrocephalus has a significant impact on global CSF absorption. A lymphatic CSF absorption deficit would appear to play some role (either direct or indirect) in the pathogenesis of ventriculomegaly.

摘要

背景

我们最近报道了在大鼠交通性脑积水高岭土模型中存在淋巴性脑脊液(CSF)吸收缺陷,脑室扩张与淋巴功能障碍的程度呈负相关。然而,如果其他部位的吸收补偿了淋巴缺陷,CSF引流可能不会有显著改变。本研究的目的是探讨淋巴吸收缺陷对整体CSF吸收(CSF流出阻力)的影响。

方法

将高岭土注入Sprague Dawley大鼠的基底池。使用磁共振成像(MRI)评估脑积水的发展。在注射后约3周的一组动物中,脑室内注射碘化人血清白蛋白(125I-HSA)向鼻甲的移动提供了CSF通过筛板进入鼻淋巴管的运输估计值(n = 18)。对照动物注射生理盐水代替高岭土(n = 10)。在高岭土注射后约3.5周的第二组动物中,在以不同流速向脊髓蛛网膜下腔输注生理盐水期间连续测量脑室内压力(n = 9)。CSF流出阻力计算为稳态压力与流速的斜率。该组的对照动物要么未注射(完整组:n = 11),要么注射生理盐水代替高岭土(n = 8)。

结果

与注射生理盐水的对照组相比,高岭土组的侧脑室体积显著更大(0.087±0.013 ml,n = 27对0.015±0.001 ml,n = 17),淋巴功能显著更低(2.14±0.72%注射量/克,n = 18对6.38±0.60%注射量/克,n = 10)。此外,高岭土组的CSF流出阻力(0.46±0.04 cm H2O μL-1 min,n = 9)显著高于注射生理盐水的组(0.28±0.03 cm H2O μL-1 min,n = 8)或完整动物组(0.18±0.03 cm H2O μL-1 min,n = 11)。CSF流出阻力与脑室体积之间存在显著正相关。

结论

数据表明,在高岭土诱导的交通性脑积水模型中,淋巴性CSF吸收障碍对整体CSF吸收有显著影响。淋巴性CSF吸收缺陷似乎在脑室扩大的发病机制中起一定作用(直接或间接)。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e82/2831828/1e0dd7d6ddec/1743-8454-7-4-1.jpg

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