康泉方对大鼠前列腺组织碱性成纤维细胞生长因子的影响
[Effect of kangquan recipe on basic fibroblast growth factor of prostate tissue in rats].
作者信息
Huang Yuan-peng, Du Jian, Hong Zhen-feng
机构信息
Zhongshan Hospital Affiliated to Xiamen University, Fujian 361004.
出版信息
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2008 Aug;28(8):729-32.
OBJECTIVE
To investigate the effect of Kangquan Recipe (KQR) on basic fibroblast growth factor (bFGF) in prostatic tissue of rats.
METHODS
Benign prostatic hyperplasia model rat was established by injecting testosterone after castration. After being administered with KQR by gastrogavage for 30 days, the model rats were killed and their abdominal lobe prostate glands were taken for determining the protein and mRNA expressions of bFGF using immunohistochemical method and RT-polymerase chain reaction (RT-PCR) respectively.
RESULTS
The expressions of bFGF and bFGF mRNA were significantly lower in the model rats being treated with high or medium dose of KQR than those in the untreated model rats (P < 0.05 or P < 0.01), but these indexes were insignificantly different between the model rats treated with low dose of KQR and untreated model rats (P > 0.05).
CONCLUSION
KQR can effectively decrease the expression of bFGF in prostatic tissue of experimental benign prostate hyperplasia model rats.
目的
探讨康泉方（KQR）对大鼠前列腺组织中碱性成纤维细胞生长因子（bFGF）的影响。
方法
去势后注射睾酮建立大鼠良性前列腺增生模型。模型大鼠经胃管灌服KQR 30天后处死，取其腹叶前列腺，分别采用免疫组化法和逆转录聚合酶链反应（RT-PCR）检测bFGF的蛋白和mRNA表达。
结果
高、中剂量KQR治疗的模型大鼠bFGF及bFGF mRNA表达均显著低于未治疗的模型大鼠（P<0.05或P<0.01），但低剂量KQR治疗的模型大鼠与未治疗的模型大鼠相比，这些指标差异无统计学意义（P>0.05）。
结论
KQR可有效降低实验性良性前列腺增生模型大鼠前列腺组织中bFGF的表达。
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