Huang Yuan-peng, Du Jian, Hong Zhen-feng
Zhongshan Hospital, Xiamen University, Fujian.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2007 Aug;27(8):711-4.
To investigate the effect of Kangquan Recipe (KQR) on apoptosis regulatory genes bax and bcl-2 mRNA in prostate of rats.
Benign prostatic hyperplasia model rat was established by injecting testosterone after castration. The model rats were killed and prostate glands were removed for examination after being treated with administration of KQR by gastrogavage for 30 days. The wet weight of prostate was measured and the mRNA expressions of bax and bcl-2 in rats' tissue of abdominal lobe of prostate were determined by RT-PCR.
Compared with the model group, wet weight of prostate was lower significantly in the groups treated with different dosages of KQR (P < 0.05 or P < 0.01), and that in the high dose KQR treated group was similar to that in the normal group (P > 0.05). Compared with the model group, the expressions of bax mRNA and ratios of bax/bcl-2 were significantly higher and the expressions of bcl-2 mRNA significantly lower in the KQR treated groups (P < 0.01), and these indexes in the high dose KQR treated group were insignificantly different from those in the normal group (P > 0.05).
KQR shows an obvious treatment effect on rats with benign prostatic hyperplasia, the mechanism might be through effectively regulating the expressions of bax mRNA and bcl-2 mRNA in prostatic tissue to accelerate the cell apoptosis of prostate in obvious dose-effect manner.
探讨康泉方（KQR）对大鼠前列腺凋亡调控基因bax和bcl-2 mRNA的影响。
去势后注射睾酮建立大鼠前列腺增生模型。模型大鼠经胃管给予KQR灌胃治疗30天后处死，摘取前列腺称重并采用逆转录聚合酶链反应（RT-PCR）法检测大鼠前列腺腹叶组织中bax和bcl-2的mRNA表达。
与模型组比较，不同剂量KQR治疗组大鼠前列腺湿重均显著降低（P<0.05或P<0.01），高剂量KQR治疗组前列腺湿重与正常组比较差异无统计学意义（P>0.05）。与模型组比较，KQR治疗组大鼠前列腺组织bax mRNA表达及bax/bcl-2比值显著升高，bcl-2 mRNA表达显著降低（P<0.01），高剂量KQR治疗组上述指标与正常组比较差异无统计学意义（P>0.05）。
KQR对大鼠前列腺增生有明显治疗作用，其机制可能是通过有效调节前列腺组织中bax mRNA和bcl-2 mRNA的表达，呈明显剂量效应关系加速前列腺细胞凋亡。
