Liu Tao, Yu Jian-chun, Han Jing-xian
Department of Acu-moxibustion, the Affiliated First Hospital of Tianjin, University of Chinese Medicine, Tianjin 300193, China.
Zhen Ci Yan Jiu. 2008 Aug;33(4):223-8, 244.
To observe the effect of acupuncture on p130 expression in neocortex and hippocampus of Senescence Accelerated Mouse/Prone (SAMP) 10 so as to explore its underlying molecular mechanism in treating dementia and aging-related diseases.
Thirty SAMP 10 were randomly divided into model, acupuncture, and non-acupoint groups with 10 cases in each, and 10 SAM/Resistant (SAMR)1 were used as normal control group. The needle manipulation of "Yi Qi Tiao Xue, Fu Ben Pei Yuan" (Reinforcing qi and blood, and strengthening the body resistance) was applied to "Shanzhong" (CV 17), "Zhongwan" (CV 12), "Qihai" (CV 16), "Xuehai" (SP 10) and "Zusanli" (ST 36), once daily for two weeks. p130 expression of neocortex and hippocampus was assayed by western blotting technique.
In SAMR 1 control group, p 130 expression in the neocortex and hippocampus exhibited an age-related stable upregulation from month 2 to 12, being significantly from the 6= month (neocortex) on and 8th month (hippocampus) on respectively (P < 0.05, 0.01). In SAMP 10 group, hippocampal and neocortical p130 expression exhibited an age-related downregulation change in the same period, fluctuating in about 6th month (downregulated remarkably in hippocampus, P < 0.01; upregulated markedly in neocortex, P < 0.01). In comparison with SAMR 1 (8 months old) group, p130 expression in hippocampus in SAMP 10 model group was upregulated significantly (P < 0.05); while that in neocortex downregulated considerably (P < 0.05). The expression of p 130 in neocortex in acupoint-acupuncture group was significantly higher than that in SAMP 10 model and non-acupoint acupuncture groups (P < 0.05). No significant differences were found between acupoint- and non-acupoint acupuncture groups in hippocampal p 130 expression, and between non-acupoint acupuncture and SAMP 10 model groups in neocortical p 130 expression (P > 0.05).
Along with the increase of aging, p 130 expression in both hippocampus and neocortex increases gradually in SAMR 1, while decreases in SAMP 10, in spite of fluctuating at month 6 in age. Acupuncture can beneficially regulate the aberrant expression of p130 in neocortex of 8-month-old SAMP 10.
观察针刺对快速老化小鼠SAMP10品系（SAMP10）大脑新皮质和海马p130表达的影响，以探讨针刺治疗痴呆及衰老相关疾病的分子机制。
将30只SAMP10小鼠随机分为模型组、针刺组和非穴位组，每组10只；另取10只抗快速老化小鼠（SAMR1）作为正常对照组。采用“益气调血，扶本培元”针法针刺“膻中”（CV17）、“中脘”（CV12）、“气海”（CV16）、“血海”（SP10）和“足三里”（ST36），每日1次，连续2周。采用蛋白质免疫印迹法检测大脑新皮质和海马p130的表达。
SAMR1正常对照组大脑新皮质和海马p130表达从2月龄至12月龄呈与年龄相关的稳定上调，分别从6月龄（新皮质）和8月龄（海马）起差异有统计学意义（P<0.05，P<0.01）。SAMP10组大脑新皮质和海马p130表达在同年龄段呈与年龄相关的下调变化，在6月龄左右出现波动（海马显著下调，P<0.01；新皮质显著上调，P<0.01）。与8月龄SAMR1组比较，SAMP10模型组海马p130表达显著上调（P<0.05），新皮质显著下调（P<0.05）。针刺组新皮质p130表达显著高于SAMP10模型组和非穴位针刺组（P<0.05）。针刺组与非穴位针刺组海马p130表达及非穴位针刺组与SAMP10模型组新皮质p130表达差异无统计学意义（P>0.05）。
随着年龄增长，SAMR1海马和新皮质p130表达逐渐增加，SAMP10则逐渐下降，6月龄时出现波动。针刺可良性调节8月龄SAMP10小鼠新皮质p130的异常表达。
