Tanji Kurenai, Bonilla Eduardo
Department of Pathology, Columbia University, New York, NY 10032, USA.
Methods. 2008 Dec;46(4):274-80. doi: 10.1016/j.ymeth.2008.09.027. Epub 2008 Oct 16.
Mitochondria are cytoplasmic, double-membrane organelles, a main role of which is to synthesize ATP, the universal energy 'supply' of cells. In the last three decades, molecular genetic, biochemical, immunological and cell biological techniques have been applied in a coordinated fashion to unveil the pathogenesis of known mitochondrial disorders, as well as to explore the role of mitochondria in aging and neurodegenerative diseases. Once to be thought to be rare, it is now clear that mitochondrial dysfunction is an important cause of neurological and cardiac diseases, and age-related disorders such as cancer. Here, we review, illustrate, and provide updated protocols of two histochemical, and three immunohistochemical methods that in our opinion are the most reliable tools to visualize mitochondria on tissue sections from normal and disease specimens.
线粒体是细胞质中的双膜细胞器，其主要作用是合成三磷酸腺苷（ATP），即细胞通用的能量“供应源”。在过去三十年中，分子遗传学、生物化学、免疫学和细胞生物学技术已被协同应用，以揭示已知线粒体疾病的发病机制，以及探索线粒体在衰老和神经退行性疾病中的作用。线粒体功能障碍曾被认为较为罕见，而现在很清楚的是，它是神经和心脏疾病以及癌症等与年龄相关疾病的重要病因。在此，我们回顾、举例说明并提供两种组织化学方法和三种免疫组织化学方法的更新方案，我们认为这些方法是在正常和疾病标本的组织切片上可视化线粒体的最可靠工具。
