Varges Renato, Marassi Carla Dray, Oelemann Walter, Lilenbaum Walter
Veterinary Bacteriology Laboratory, Universidade Federal Fluminense, Niterói 24210-130, Brazil.
Res Vet Sci. 2009 Jun;86(3):371-2. doi: 10.1016/j.rvsc.2008.08.006. Epub 2008 Oct 18.
In this experiment 63 animals from a paratuberculosis (PTB) and tuberculosis-free herd were tested by Intradermal Tuberculin Tests (ITT) and blood samples were collected before PPD inoculation and on days 3, 15, 30, 60 and 90 post-inoculation (p.i.). Sera were tested for PTB-specific antibodies by ELISA-PPA and confirmed by a commercial ELISA. Three (4.76%) animals were positive by ELISA-PPA and five (7.93%) in the commercial ELISA, between days 30 and 90 p.i. These results suggest that ITT can interfere in the reliability of ELISAs and that serological testing for PTB should be avoided for 90d after PPD inoculation.
在本实验中，对来自无副结核病（PTB）和结核病牛群的63只动物进行了皮内结核菌素试验（ITT），并在接种PPD之前以及接种后第3、15、30、60和90天采集血样。通过酶联免疫吸附试验-聚丙烯酸（ELISA-PPA）检测血清中PTB特异性抗体，并通过商业ELISA进行确认。在接种后第30至90天之间，ELISA-PPA检测出3只（4.76%）动物呈阳性，商业ELISA检测出5只（7.93%）动物呈阳性。这些结果表明，ITT可能会干扰ELISA的可靠性，并且在接种PPD后90天内应避免进行PTB的血清学检测。
