Fisker Hag Anne Mette, Pedersen Sune Folke, Kjaer Andreas
Cluster for Molecular Imaging, Faculty of Health Science, University of Copenhagen, Blegdamsvej 3B, BMI. 12.3.15, DK-2200 Copenhagen N, and Department of Clinical Physiology, Nuclear Medicine & PET, Rigshospitalet, Denmark.
Cluster for Molecular Imaging, Faculty of Health Science, University of Copenhagen, Blegdamsvej 3B, BMI. 12.3.15, DK-2200 Copenhagen N, and Department of Clinical Physiology, Nuclear Medicine & PET, Rigshospitalet, Denmark.
Biochem Biophys Res Commun. 2008 Dec 12;377(2):689-693. doi: 10.1016/j.bbrc.2008.10.037. Epub 2008 Oct 18.
To identify markers of the earliest stage of atherosclerosis, endothelial dysfunction, we evaluated the gene expression of lectin-like oxidized-low-density-lipoprotein receptor-1 (LOX-1), vascular cell adhesion molecule-1 (VCAM-1), and intercellular adhesion molecule-1 (ICAM-1) in very young pre-atherosclerotic mice. Furthermore, the plasma levels of the soluble VCAM-1 and ICAM-1 were compared to the gene expression profiles. Gene expressions of LOX-1 and VCAM-1 were up-regulated in young apoE(-/-) mice, and thus, it seems probable that these genes play a role in pre-atherosclerosis. Contrarily, the gene expression profile of ICAM-1 did not show any apparent differences between the groups, questioning the involvement of this molecule in the early development of atherosclerosis. Plasma levels of sVCAM-1 and sICAM-1 were similar in all mice and did not correlate with the vascular gene expression of the corresponding genes. It therefore seems likely that these circulating markers are not suited to detect early atherosclerosis.
为了识别动脉粥样硬化最早阶段的标志物——内皮功能障碍，我们评估了极年幼的动脉粥样硬化前期小鼠中凝集素样氧化低密度脂蛋白受体-1（LOX-1）、血管细胞黏附分子-1（VCAM-1）和细胞间黏附分子-1（ICAM-1）的基因表达。此外，还将可溶性VCAM-1和ICAM-1的血浆水平与基因表达谱进行了比较。LOX-1和VCAM-1的基因表达在年幼的载脂蛋白E基因敲除（apoE(-/-)）小鼠中上调，因此，这些基因似乎可能在动脉粥样硬化前期起作用。相反，ICAM-1的基因表达谱在各组之间未显示出任何明显差异，这对该分子参与动脉粥样硬化早期发展提出了质疑。所有小鼠中可溶性血管细胞黏附分子-1（sVCAM-1）和可溶性细胞间黏附分子-1（sICAM-1）的血浆水平相似，且与相应基因的血管基因表达无关。因此，这些循环标志物似乎不太适合检测早期动脉粥样硬化。
