Parrella Edoardo, Longo Valter D
Andrus Gerontology Center and Department of Biological Sciences, University of Southern California, 3715 McClintock Avenue, Los Angeles, CA 90089-0191, USA.
Methods. 2008 Dec;46(4):256-62. doi: 10.1016/j.ymeth.2008.10.004. Epub 2008 Oct 18.
Saccharomyces cerevisiae has played an important role as a model system to understand the biochemistry and molecular biology of mammalian cells. The genetic tools available and the short life span have also made S. cerevisiae a powerful system to study aging. The yeast chronological life span (CLS) is a measure of the survival of a non-dividing population of cells, and thus can model aging of mammalian non-dividing cells but also of higher eukaryotic organisms. The parallel description of the pro-aging role of homologs of Akt, S6 kinase, adenylate cyclase, and Tor in yeast and in higher eukaryotes, suggests that findings in the S. cerevisiae will be valuable to understand human aging and diseases. Moreover, the similarities between mitochondria and age-dependent mitochondrial damage in yeast and mammalian cells indicate that S. cerevisiae is a valuable model to study mitochondrial dysfunction and diseases that involve this organelle. Here, we describe the use of S. cerevisiae CLS in combination with three methods to quantify age-dependent mitochondrial damage and the accumulation of mitochondrial DNA mutations.
酿酒酵母作为一种模型系统，在理解哺乳动物细胞的生物化学和分子生物学方面发挥了重要作用。可用的遗传工具以及较短的寿命也使酿酒酵母成为研究衰老的强大系统。酵母的时序寿命（CLS）是衡量非分裂细胞群体存活能力的指标，因此不仅可以模拟哺乳动物非分裂细胞的衰老，还能模拟高等真核生物的衰老。对酵母和高等真核生物中Akt、S6激酶、腺苷酸环化酶和Tor同源物的促衰老作用的平行描述表明，在酿酒酵母中的研究结果对于理解人类衰老和疾病具有重要价值。此外，酵母和哺乳动物细胞中线粒体与年龄相关的线粒体损伤之间的相似性表明，酿酒酵母是研究线粒体功能障碍以及涉及该细胞器的疾病的有价值模型。在此，我们描述了将酿酒酵母CLS与三种方法结合使用，以量化与年龄相关的线粒体损伤和线粒体DNA突变的积累。
