酿酒酵母的自然寿命。

The chronological life span of Saccharomyces cerevisiae.

作者信息

Fabrizio Paola, Longo Valter D

机构信息

Adrus Gerontology Center, Division of Biogerentology and Department of Biological Sciences, University of Southern California, Los Angeles, CA, USA.

出版信息

Methods Mol Biol. 2007;371:89-95. doi: 10.1007/978-1-59745-361-5_8.

DOI:10.1007/978-1-59745-361-5_8

PMID:17634576

Abstract

The chronological life span of yeast, which is measured as the survival time of populations of nondividing cells, has been used successfully for the identification of key pathways responsible for the regulation of aging. These pathways have remarkable similarities with those that regulate the life span in higher eukaryotes, suggesting that longevity depends on the activity of genes and signaling pathways that share a common evolutionary origin. Thus, the unicellular Saccharomyces cerevisiae is a simple model system that can provide significant insights into the human genetics and molecular biology of aging. Here, we describe the standard procedures to measure the chronological life span, including both the normal and calorie restriction paradigms.

摘要

酵母的时序寿命是以不分裂细胞群体的存活时间来衡量的，它已成功用于识别负责衰老调控的关键途径。这些途径与调控高等真核生物寿命的途径有显著相似之处，这表明寿命取决于具有共同进化起源的基因和信号通路的活性。因此，单细胞酿酒酵母是一个简单的模型系统，能够为人类衰老的遗传学和分子生物学提供重要见解。在这里，我们描述了测量时序寿命的标准程序，包括正常和卡路里限制模式。