Conesa-Zamora P, Doménech-Peris A, Ortiz-Reina S, Orantes-Casado F J, Acosta-Ortega J, García-Solano J, Pérez-Guillermo M
Grupo de Patología Molecular y Farmacogenética, Hospital Universitario Santa María del Rosell, Cartagena, Spain.
J Clin Pathol. 2009 Feb;62(2):159-62. doi: 10.1136/jcp.2008.061408. Epub 2008 Oct 17.
Considering the sparse information about the clinical utility of the novel immunohistochemical marker ProEx C in histological sections, a decision was taken to study the pattern of ProEx C expression in normal/benign cervical epithelium (N/B), low-grade squamous intraepithelial lesion (LGSIL) and high-grade squamous intraepithelial lesion (HGSIL), as well as the association of ProEx C expression with human papillomavirus (HPV) genotypes.
100 cervical samples, including 21 N/B cervices, 16 LGSILs, 61 HGSILs and two cervical invasive carcinomas, were obtained from conisation and hysterectomy. Surgical specimens were arranged in three tissue microarrays and stained for ProEx C. Ninety-three samples were HPV genotyped. Genotyping was performed by DNA amplification and hybridisation with genotype-specific probes on a low-density DNA array.
ProEx C-positive expression in more than the lower third of the epithelium was observed in 14.3% of N/B, 62.5% of LGSIL and 90.2% of HGSIL. Seventy percent of HPV positivity was found in cases with expression in more than the lower third of the epithelium. Of 31 cases that were positive for HPV16, 16.1% showed ProEx C expression restricted to one or two basal layers, and 83.9% showed ProEx C expression in more than the lower third of the epithelium.
ProEx C is significantly associated with HPV16 infection and is a useful adjunct in the identification of LGSIL and HGSIL in histological sections when expressed in more than the lower third of the epithelium.
鉴于新型免疫组化标志物ProEx C在组织切片中的临床应用信息匮乏，决定研究ProEx C在正常/良性宫颈上皮（N/B）、低级别鳞状上皮内病变（LGSIL）和高级别鳞状上皮内病变（HGSIL）中的表达模式，以及ProEx C表达与人乳头瘤病毒（HPV）基因型的关联。
从锥切术和子宫切除术中获取100份宫颈样本，包括21份N/B宫颈样本、16份LGSIL样本、61份HGSIL样本和两份宫颈浸润癌样本。手术标本被制作成三个组织微阵列，并进行ProEx C染色。对93份样本进行HPV基因分型。基因分型通过DNA扩增以及在低密度DNA阵列上与基因型特异性探针杂交来进行。
在14.3%的N/B样本、62.5%的LGSIL样本和90.2%的HGSIL样本中观察到上皮下三分之一以上区域有ProEx C阳性表达。在上皮下三分之一以上区域有表达的病例中，70%为HPV阳性。在31例HPV16阳性病例中，16.1%的病例ProEx C表达局限于一或两个基底层，83.9%的病例ProEx C表达在上皮下三分之一以上区域。
ProEx C与HPV16感染显著相关，当在上皮下三分之一以上区域表达时，它是组织切片中鉴别LGSIL和HGSIL的有用辅助指标。
