Booss J, Suard I, Collins P V, Jacque C
INSERM U-134, Hôpital de la Salpêtrière, Paris, France.
Brain Res. 1991 May 17;549(1):19-24. doi: 10.1016/0006-8993(91)90594-l.
Following transplantation of fragments of embryonic rabbit brain into the brains of newborn mice, the proportion of mice bearing detectable xenogenic astrocytes increases to over 80% in the first 3-4 weeks. Recent studies have demonstrated that the host response at this time was dominated by non-specific elements of host defense: macrophages, microglia and astrocytes. In the second phase, the proportion of mice bearing xenogenic astrocytes declines rapidly after 4 weeks and reaches zero by 10 weeks. In the present experiments, designed to characterize the host defense during this period, a dramatic increase in the proportion of mice displaying T-cells in the brain in the fourth and fifth weeks after transplantation was found. This corresponded with a marked decline of xenogenic astrocytes. Both subsets of T-cell, helper-inducer (L3T4) and cytotoxic-suppressor (Lyt2), were found, with L3T4 more numerous in many samples. T-cells were found at the site of transplantation and at sites of migration. The division of the host-defense response in this model into a phase of antigen non-specific cells followed by a period when T-cells appear and transplanted astrocytes disappear, should facilitate kinetic studies into the mechanisms of brain-graft rejection.
将胚胎兔脑片段移植到新生小鼠脑内后，在最初3 - 4周内，带有可检测到的异种星形胶质细胞的小鼠比例增加到80%以上。最近的研究表明，此时宿主反应主要由宿主防御的非特异性成分主导：巨噬细胞、小胶质细胞和星形胶质细胞。在第二阶段，带有异种星形胶质细胞的小鼠比例在4周后迅速下降，到10周时降至零。在本实验中，为了表征这一时期的宿主防御，发现在移植后第四和第五周，脑内显示T细胞的小鼠比例急剧增加。这与异种星形胶质细胞的显著减少相对应。发现了T细胞的两个亚群，辅助诱导型（L3T4）和细胞毒性抑制型（Lyt2），在许多样本中L3T4数量更多。在移植部位和迁移部位发现了T细胞。在这个模型中，宿主防御反应分为抗原非特异性细胞阶段，随后是T细胞出现且移植的星形胶质细胞消失的时期，这应该有助于对脑移植排斥机制进行动力学研究。
