Zhang J Z, Chen D Y, Wu S, Harke H R, Dovichi N J
Department of Chemistry, University of Alberta, Edmonton, Canada.
Clin Chem. 1991 Sep;37(9):1492-6.
Capillary electrophoresis provides very high efficiency separations of biological molecules. Laser-induced fluorescence produces very high sensitivity detection. The combination of the two techniques results in an analytical tool with unprecedented properties: separations with more than a million theoretical plates and detection limits of a few hundred analyte molecules. This paper considers the design of high-sensitivity laser-induced fluorescence detection for capillary zone electrophoresis separation of labeled amino acids and capillary gel electrophoresis separation of DNA sequencing fragments.
毛细管电泳可对生物分子进行高效分离。激光诱导荧光可实现高灵敏度检测。这两种技术相结合，形成了一种具有前所未有的特性的分析工具：能实现超过百万理论塔板数的分离，且对几百个分析物分子具有检测限。本文探讨了用于标记氨基酸的毛细管区带电泳分离以及DNA测序片段的毛细管凝胶电泳分离的高灵敏度激光诱导荧光检测的设计。
