Timperman A T, Khatib K, Sweedler J V
Department of Chemistry, University of Illinois, Urbana 61801.
Anal Chem. 1995 Jan 1;67(1):139-44. doi: 10.1021/ac00097a022.
A multichannel laser-induced fluorescence detector for capillary electrophoresis is described. The detection system combines yoctomole limits of detection with the simultaneous acquisition of entire fluorescence emission spectra. An Ar/Kr mixed-gas ion laser provides great flexibility in excitation wavelengths, and a holographic grating and charge-coupled device detector combination allows a 500-nm spectral window to be acquired with 2-nm resolution. The limits of detection are 5 x 10(-14) M or 80 molecules for sulforhodamine 101 and 1.5 x 10(-13) M or 220 molecules for fluorescein in a 50 micron i.d. capillary. An electropherogram of a mixture of amino acids derivatized with both Bodipy 503/512 C3 and Bodipy 576/589 C3 demonstrates that the analytes can be differentiated on the basis of both emission characteristics and migration times. With the use of organic modifiers and other complex separation media, the spectral background increases as discrete spectral features appear; the wavelength-resolved backgrounds of a variety of common CE separation conditions are presented.
本文描述了一种用于毛细管电泳的多通道激光诱导荧光检测器。该检测系统将检测限低至 yoctomole 与同时采集整个荧光发射光谱相结合。氩/氪混合气体离子激光器在激发波长方面提供了极大的灵活性，全息光栅和电荷耦合器件检测器的组合使得能够以 2 nm 的分辨率采集 500 nm 的光谱窗口。对于内径为 50 微米的毛细管中的磺基罗丹明 101，检测限为 5×10⁻¹⁴ M 或 80 个分子；对于荧光素，检测限为 1.5×10⁻¹³ M 或 220 个分子。用 Bodipy 503/512 C3 和 Bodipy 576/589 C3 衍生化的氨基酸混合物的电泳图表明，分析物可以根据发射特性和迁移时间进行区分。随着有机改性剂和其他复杂分离介质的使用，随着离散光谱特征的出现，光谱背景增加；本文给出了各种常见毛细管电泳分离条件下的波长分辨背景。
