Winkler T H, Jahn S, Kalden J R
Department of Medicine III, University Erlangen-Nuernberg, Germany.
Clin Exp Immunol. 1991 Sep;85(3):379-85. doi: 10.1111/j.1365-2249.1991.tb05735.x.
We describe the production of six mouse-human heterohybridomas secreting human IgG anti-dsDNA antibodies derived from patients with systemic lupus erythematosus (SLE). Peripheral blood cells used for fusion experiments were from patients who were shown to have high numbers of anti-DNA secreting B cells in the peripheral blood. All monoclonal antibodies bind to dsDNA in ELISA systems, five are reactive with Crithidia lucilae kinetoplasts and three precipitate dsDNA in the Farr assay. Inhibition studies revealed a remarkable specificity for certain polynucleotide structures. To our knowledge these are the first hybridomas described in the human system that secrete anti-dsDNA antibodies of the IgG class.
我们描述了六株小鼠 - 人异源杂交瘤的产生，这些杂交瘤分泌源自系统性红斑狼疮（SLE）患者的人IgG抗双链DNA抗体。用于融合实验的外周血细胞来自那些在外周血中显示有大量分泌抗DNA B细胞的患者。所有单克隆抗体在ELISA系统中均与双链DNA结合，五种与利什曼原虫动基体反应，三种在Farr试验中沉淀双链DNA。抑制研究揭示了对某些多核苷酸结构的显著特异性。据我们所知，这些是人类系统中描述的首批分泌IgG类抗双链DNA抗体的杂交瘤。
