人体指甲的胶带剥离：去除量的量化

Tape stripping on a human nail: quantification of removal.

作者信息

Tudela E, Lamberbourg A, Cordoba Diaz M, Zhai H, Maibach H I

机构信息

Department of Dermatology, University of California San Francisco, San Francisco, CA 9143-0989, USA.

出版信息

Skin Res Technol. 2008 Nov;14(4):472-7. doi: 10.1111/j.1600-0846.2008.00317.x.

DOI:10.1111/j.1600-0846.2008.00317.x

PMID:18937784

Abstract

BACKGROUND/PURPOSE: Tape stripping is commonly used to investigate the stratum corneum (SC). This study assesses if protein quantitative tape stripping method was suitable for human nails.

METHOD

We used a colorimetric method to quantify proteins removed by the tape. Water barrier functions as a result of tape stripping were also observed by changes in transonychial water loss (TOWL) from the baseline.

RESULTS

Using tape stripping, we observed no difference between nails in the protein quantity removed by tape stripping (P=0.39). The mean TOWL before and after tape stripping were 6.9 and 9.3 g/m2/h, respectively; this was significantly increased in tape stripped nails (P<0.0001).

CONCLUSION

Tape stripping seems to be an effective method to extract proteins from human nail plate and may aid the study of nail structure and function. Further studies are needed to extend our results in terms of age, gender, ethnicity and disease.

摘要

背景/目的：胶带剥离法常用于研究角质层（SC）。本研究评估蛋白质定量胶带剥离法是否适用于人类指甲。