Tudela E, Lamberbourg A, Cordoba Diaz M, Zhai H, Maibach H I
Department of Dermatology, University of California San Francisco, San Francisco, CA 9143-0989, USA.
Skin Res Technol. 2008 Nov;14(4):472-7. doi: 10.1111/j.1600-0846.2008.00317.x.
BACKGROUND/PURPOSE: Tape stripping is commonly used to investigate the stratum corneum (SC). This study assesses if protein quantitative tape stripping method was suitable for human nails.
We used a colorimetric method to quantify proteins removed by the tape. Water barrier functions as a result of tape stripping were also observed by changes in transonychial water loss (TOWL) from the baseline.
Using tape stripping, we observed no difference between nails in the protein quantity removed by tape stripping (P=0.39). The mean TOWL before and after tape stripping were 6.9 and 9.3 g/m2/h, respectively; this was significantly increased in tape stripped nails (P<0.0001).
Tape stripping seems to be an effective method to extract proteins from human nail plate and may aid the study of nail structure and function. Further studies are needed to extend our results in terms of age, gender, ethnicity and disease.
背景/目的：胶带剥离法常用于研究角质层（SC）。本研究评估蛋白质定量胶带剥离法是否适用于人类指甲。
我们采用比色法对胶带去除的蛋白质进行定量。还通过测量脱甲水分流失（TOWL）相对于基线的变化，观察胶带剥离导致的水屏障功能变化。
使用胶带剥离法，我们观察到不同指甲之间胶带剥离去除的蛋白质量没有差异（P = 0.39）。胶带剥离前后的平均TOWL分别为6.9和9.3 g/m2/h；胶带剥离后的指甲中TOWL显著增加（P < 0.0001）。
胶带剥离似乎是从人类指甲板中提取蛋白质的有效方法，可能有助于指甲结构和功能的研究。需要进一步研究以在年龄、性别、种族和疾病方面扩展我们的研究结果。
