Dreher F, Arens A, Hostýnek J J, Mudumba S, Ademola J, Maibach H I
School of Medicine, Department of Dermatology, University of California, San Francisco 94143, USA.
Acta Derm Venereol. 1998 May;78(3):186-9. doi: 10.1080/000155598441495.
Tape-stripping of the skin is a useful method for removing the stratum corneum and obtaining more information about the function of this skill layer as the main barrier for skin penetration. The amount of stratum corneum removed is of relevance in establishing the concentration profile of chemicals within the stratum corneum after topical application. Weighing is the preferred method for measuring the amount stripped, but because it is often subject to artifacts, alternative methods are sought. We present a simple, colorimetric method for determining the amount stratum corneum removed by sequential adhesive-tape-stripping of human skin in vivo. The method is based on quantification of the sodium hydroxide soluble protein fraction using a commercially available protein assay similar to the Lowry assay. The method is shown to be an accurate and reproducible alternative to weighing, also demonstrating uniform removal of stratum corneum layers following the very initial strips.
皮肤胶带剥离法是一种有用的方法，可用于去除角质层，并获取有关该皮肤层功能的更多信息，因为它是皮肤渗透的主要屏障。去除的角质层量对于确定局部应用后化学物质在角质层内的浓度分布具有重要意义。称重是测量剥离量的首选方法，但由于它经常受到假象的影响，因此人们在寻找替代方法。我们提出了一种简单的比色法，用于在体内通过连续胶带剥离人体皮肤来确定去除的角质层量。该方法基于使用类似于洛瑞法的市售蛋白质测定法对氢氧化钠可溶性蛋白质部分进行定量。结果表明，该方法是一种准确且可重复的称重替代方法，还表明在最初的几条胶带之后角质层各层被均匀去除。
