Metian M, Warnau M, Cosson R P, Oberhänsli F, Bustamante P
International Atomic Energy Agency - Marine Environment Laboratories, 4 Quai Antoine Ier, MC-98000, Principality of Monaco.
Aquat Toxicol. 2008 Nov 21;90(3):204-13. doi: 10.1016/j.aquatox.2008.08.014. Epub 2008 Sep 5.
Hg bioaccumulation was investigated in the king scallop Pecten maximus in the laboratory and in the field. In controlled conditions, scallops were exposed to (203)Hg through seawater, sediment and food in order to determine its uptake and depuration kinetics. In the field, Hg and metallothionein (MT) concentrations and the metal subcellular distribution were determined in scallops from two sites of the Bay of Seine (France) differently subjected to the Seine river inputs. While Hg concentrations in the whole soft parts and kidneys (viz. the highest accumulator organ) did not differ between scallops from both sites (74-156 ng g(-1)dry wt), they did for the digestive gland and the gills. According to the experimental results, a higher exposure to dissolved Hg might occur in the site close to the estuary whereas Hg would be mainly incorporated via the dietary pathway in the site away from the estuary. Within the cells of wild scallops, Hg was mainly associated to the cytosolic fraction in the digestive gland and gills (60-100%). However, the lack of relationship between Hg and MT levels suggests that Hg detoxification in P. maximus involves other, non-MT, soluble compounds. In kidneys, insoluble compounds played an important role in Hg sequestration. No effect of scallop age was observed neither on Hg and MT concentrations nor on the subcellular distribution of the metal. Finally, according to FAO/WHO recommendations (maximum weekly Hg intake), our results clearly indicate that the low Hg contents in the edible part of the king scallops from the Bay of Seine prevent any risk for human consumers.
在实验室和野外对大扇贝（Pecten maximus）中的汞生物积累情况进行了研究。在可控条件下，让扇贝通过海水、沉积物和食物接触汞（203Hg），以确定其吸收和净化动力学。在野外，测定了来自法国塞纳湾两个受塞纳河输入影响程度不同地点的扇贝中的汞和金属硫蛋白（MT）浓度以及金属的亚细胞分布。虽然两个地点的扇贝整个软体部分和肾脏（即汞积累量最高的器官）中的汞浓度没有差异（74 - 156 ng g(-1)干重），但消化腺和鳃中的汞浓度存在差异。根据实验结果，靠近河口的地点可能接触到更高浓度的溶解汞，而远离河口的地点汞主要通过饮食途径摄入。在野生扇贝细胞内，汞主要与消化腺和鳃中的胞质部分结合（60 - 100%）。然而，汞与MT水平之间缺乏相关性表明，大扇贝中的汞解毒涉及其他非MT可溶性化合物。在肾脏中，不溶性化合物在汞的螯合中起重要作用。未观察到扇贝年龄对汞和MT浓度以及金属亚细胞分布有影响。最后，根据粮农组织/世界卫生组织的建议（每周最大汞摄入量），我们的结果清楚地表明，塞纳湾大扇贝可食用部分的低汞含量对人类消费者没有任何风险。
