de Kloet Siwo R
Animal Genetics Inc., 1336 Timberlane Road, Tallahassee, FL 32312, USA.
Avian Dis. 2008 Sep;52(3):480-6. doi: 10.1637/8212-011908-Reg.1.
This paper describes the characterization of four double-stranded ribonucleic acid segments, S1, S2, S3, and S4, of a newly identified pathogenic reovirus from parrots. The four segments share a unique 5' terminus GCUUUUC. The amino-acid sequences of the conserved sigma A and sigma NS proteins show less than 60% sequence similarity, whereas those of the outer capsid proteins sigma B and sigma C have at most 47% sequence similarity to their counterparts in other bird or bat reoviruses. In a phylogenetic analysis of the amino-acid sequences, the proteins coded for by the S1 segment, P10, P17, and sigma C, group with their homologous proteins in other avian reoviruses, whereas the major capsid protein, sigma B, and the nonstructural protein, sigma NS, show more sequence similarity to their bat reoviral counterparts. The phylogenetic relationship of sigma A with the homologous avian and bat sequences is unresolved. The possibility that the parrot reovirus has evolved from an ancestral, more batlike reovirus is discussed. It is proposed to designate this unique virus as PsRV.
本文描述了一种新鉴定的来自鹦鹉的致病性呼肠孤病毒的四个双链核糖核酸片段S1、S2、S3和S4的特征。这四个片段共享一个独特的5'末端GCUUUUC。保守的σA和σNS蛋白的氨基酸序列显示出小于60%的序列相似性，而外衣壳蛋白σB和σC与其他鸟类或蝙蝠呼肠孤病毒中的对应蛋白的序列相似性最高为47%。在氨基酸序列的系统发育分析中，S1片段编码的蛋白P10、P17和σC与其他禽呼肠孤病毒中的同源蛋白归为一类，而主要衣壳蛋白σB和非结构蛋白σNS与它们的蝙蝠呼肠孤病毒对应蛋白显示出更多的序列相似性。σA与同源禽类和蝙蝠序列的系统发育关系尚未确定。讨论了鹦鹉呼肠孤病毒可能从更类似蝙蝠的祖先呼肠孤病毒进化而来的可能性。建议将这种独特的病毒命名为PsRV。
