Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY, USA.
Marine Animal Disease Laboratory, College of Veterinary Medicine, University of Florida, Gainesville, FL, USA.
J Gen Virol. 2011 Nov;92(Pt 11):2558-2565. doi: 10.1099/vir.0.032649-0. Epub 2011 Jul 27.
An aborted mid-gestational male Steller sea lion fetus with an attached placenta was recovered on the floor of an open floating capture trap located off Norris Rock near Denman Island, British Columbia. Viral culture of the placenta demonstrated cytopathic effect. Although no specific signal was obtained in microarray experiments using RNA obtained from viral culture, elution and sequence analysis revealed the presence of a reovirus. Complete genome pyrosequencing led to the identification of an orthoreovirus that we have tentatively named Steller sea lion reovirus (SSRV). Phylogenetic analysis revealed similarities between SSRV and orthoreoviruses of birds, bats and other mammals that suggests potential for interspecies transmission.
在不列颠哥伦比亚省德门岛附近的诺里斯岩外的开放式浮动捕捞陷阱底部，发现了一只中途流产的雄性斯特勒海狮胎儿，其胎盘仍附着在上面。胎盘的病毒培养显示出细胞病变效应。虽然从病毒培养物中获得的 RNA 进行微阵列实验未获得特定信号，但洗脱和序列分析显示存在呼肠孤病毒。完整基因组焦磷酸测序导致鉴定出一种正呼肠孤病毒，我们暂定名为海狮呼肠孤病毒（SSRV）。系统进化分析显示，SSRV 与鸟类、蝙蝠和其他哺乳动物的正呼肠孤病毒具有相似性，表明存在种间传播的可能性。
