Johansson Lina M, Johansson Lovisa E, Ridderstråle Martin
Department of Clinical Sciences Malmö, Clinical Obesity, Lund University, University Hospital MAS, 205 02 Malmö, Sweden.
Metabolism. 2008 Nov;57(11):1558-62. doi: 10.1016/j.metabol.2008.06.011.
The newly discovered adipokine visfatin has been hypothesized to be related to obesity and insulin resistance. In this study, we investigate if the 2 single nucleotide polymorphisms rs4730153 and G-948T are associated with obesity and/or related traits and whether they influence the messenger RNA (mRNA) levels of PBEF1 (originally the abbreviation for pre-B-cell colony-enhancing factor 1) in visceral and subcutaneous adipose tissue (VAT and SAT). We found that obese carriers of the PBEF1 G-948T variant allele had significantly higher levels of high-density lipoprotein cholesterol (GG, 1.1 [0.97-1.3] mmol/L; GT + TT, 1.3 [1.0-1.5] mmol/L; P = .02). Other than that, neither rs4730153 nor G-948T had any major impact on any of the obesity-related phenotypes. There was no difference in mRNA expression between VAT and SAT (2.08 +/- 0.17 and 2.09 +/- 0.14, respectively; P = .26), but there was a nonsignificant trend toward higher PBEF1 mRNA levels in the variant allele carriers concerning both VAT and SAT for both single nucleotide polymorphisms. A significant correlation was observed between body mass index and PBEF1 mRNA expression in SAT (R = 0.37, P = .03) but not in VAT (R = 0.26, P = .12). In conclusion, PBEF1 G-948T is associated with increased high-density lipoprotein cholesterol; but genetic variation in PBEF1 does not seem to have a major impact on the development of obesity or on the expression of the gene.
新发现的脂肪因子内脂素被认为与肥胖和胰岛素抵抗有关。在本研究中，我们调查了两个单核苷酸多态性rs4730153和G-948T是否与肥胖及相关性状相关，以及它们是否影响内脏和皮下脂肪组织（VAT和SAT）中PBEF1（原B细胞集落增强因子1的缩写）的信使核糖核酸（mRNA）水平。我们发现，携带PBEF1 G-948T变异等位基因的肥胖者的高密度脂蛋白胆固醇水平显著更高（GG型，1.1[0.97 - 1.3]mmol/L；GT + TT型，1.3[1.0 - 1.5]mmol/L；P = 0.02）。除此之外，rs4730153和G-948T对任何肥胖相关表型均无重大影响。VAT和SAT之间的mRNA表达没有差异（分别为2.08±0.17和2.09±0.14；P = 0.26），但对于这两个单核苷酸多态性，变异等位基因携带者的VAT和SAT中PBEF1 mRNA水平均有不显著的升高趋势。在SAT中观察到体重指数与PBEF1 mRNA表达之间存在显著相关性（R = 0.37，P = 0.03），而在VAT中未观察到（R = 0.26，P = 0.12）。总之，PBEF1 G-948T与高密度脂蛋白胆固醇升高有关；但PBEF1的基因变异似乎对肥胖的发展或该基因的表达没有重大影响。
