Phytopathology. 2005 Jul;95(7):834-9. doi: 10.1094/PHYTO-95-0834.
ABSTRACT A quantitative trait loci (QTL) analysis of resistance to Sclerotinia sclerotiorum was carried out with 283 sunflower (Helianthus annuus) F(2:3) families derived from a cross between a resistant (SWS-B-04) and a highly susceptible sunflower inbred line. For that purpose, a genetic map based on 195 amplified fragment length polymorphism and 20 simple sequence repeat markers was constructed. The map has a size of 2,273.5 centimorgans and comprises 17 linkage groups, 12 of which could be associated to already defined linkage groups. The heads of sunflower F(3) families were artificially inoculated by using sclerotinia mycelium in three field environments. The lesion length was measured in centimeters 1 week postinoculation and head rot was scored according to a 1-to-8 head rot scale 2 weeks postinoculation. Using the composite interval mapping procedure, three QTL for lesion length and two QTL for head rot could be identified. These QTL explain 10.6 to 17.1% of the total phenotypic variance.
利用源自抗 Sclerotinia sclerotiorum 的（SWS-B-04）和高度易感的向日葵自交系杂交的 283 个向日葵 F(2:3)家系，进行了对 Sclerotinia sclerotiorum 抗性的数量性状位点（QTL）分析。为此，构建了基于 195 个扩增片段长度多态性和 20 个简单序列重复标记的遗传图谱。图谱大小为 2273.5 厘摩，包含 17 个连锁群，其中 12 个可以与已经定义的连锁群相关联。在三个田间环境中，通过使用菌核霉菌丝人工接种向日葵 F(3)家系的头部。接种后 1 周，以厘米为单位测量病变长度，并根据接种后 2 周的 1 到 8 级头部腐烂量表对头部腐烂进行评分。使用复合区间作图程序，可以鉴定出三个与病变长度相关的 QTL 和两个与头部腐烂相关的 QTL。这些 QTL 解释了总表型方差的 10.6%至 17.1%。
