Kallamadi Prathap Reddy, Mulpuri Sujatha
ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad, Telangana 500 030 India.
3 Biotech. 2020 May;10(5):234. doi: 10.1007/s13205-020-02224-2. Epub 2020 May 6.
Sources of resistance to powdery mildew incited by have been identified in wild sunflowers and few exotic lines. The present investigation has been undertaken to study the inheritance of powdery mildew resistance and to map the quantitative trait loci (QTLs) governing resistance to powdery mildew in a multiple disease resistance line, TX16R (PI 642072). The inheritance was observed as a continuous distribution in a set of 264 F population and 93 recombinant inbred lines (RILs) of a cross between a highly susceptible accession PS 2023 and TX16R. Screening of the two population sets was done with 484 sunflower-specific SSR primers of which 175 primers showed polymorphism between the parents. Based on the phenotyping and genotyping data, the linkage map was constructed with 93 RILs. The map spanned 1200 cM and included 64 markers distributed along the 17 sunflower chromosomes in the haploid set. Quantitative trait loci (QTL) analysis identified three genomic regions for resistance to powdery mildew, two of which mapped on chromosome 10 and one on chromosome 5. This is the first report on mapping of powdery mildew resistance in sunflower and paves the way in fine mapping and introgression of resistance for powdery mildew in sunflower through marker-assisted breeding.
在野生向日葵和少数外来品系中已鉴定出对白粉病的抗性来源。本研究旨在研究白粉病抗性的遗传，并在一个多病害抗性品系TX16R（PI 642072）中定位控制白粉病抗性的数量性状基因座（QTL）。在一组264个F群体和一个高感材料PS 2023与TX16R杂交的93个重组自交系（RIL）中，观察到抗性呈连续分布。用484个向日葵特异性SSR引物对这两个群体进行筛选，其中175个引物在亲本间表现出多态性。基于表型和基因分型数据，用93个RIL构建了连锁图谱。该图谱跨度为1200 cM，包含64个标记，分布在单倍体组的17条向日葵染色体上。数量性状基因座（QTL）分析确定了三个白粉病抗性基因组区域，其中两个位于第10号染色体上，一个位于第5号染色体上。这是关于向日葵白粉病抗性图谱构建的首次报道，为通过标记辅助育种对向日葵白粉病抗性进行精细定位和导入奠定了基础。
