Lu Yan, Huang Jiaying, Liu Dongqi, Kong Xiangjiu, Song Yang, Jing Lan
College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Huhhot 010011, China.
Life (Basel). 2024 Oct 17;14(10):1322. doi: 10.3390/life14101322.
The sunflower, an important oilseed crop and food source across the world, is susceptible to several pathogens, which cause severe losses in sunflower production. The utilization of genetic resistance is the most economical, effective measure to prevent infectious diseases. Based on the sunflower pangenome, in this study, we explored the variability of resistance gene analogs (RGAs) within the species. According to a comparative analysis of RGA candidates in the sunflower pangenome using the RGAugury pipeline, a total of 1344 RGAs were identified, comprising 1107 conserved, 199 varied, and 38 rare RGAs. We also identified RGAs associated with resistance against () in sunflower at the quantitative trait locus (QTL). A total of 61 RGAs were found to be located at four quantitative trait loci (QTLs). Through a detailed expression analysis of RGAs in one susceptible and two tolerant sunflower inbred lines (ILs) across various time points post inoculation, we discovered that 348 RGAs exhibited differential expression in response to Sclerotinia head rot (SHR), with 17 of these differentially expressed RGAs being situated within the QTL regions. In addition, 15 RGA candidates had gene introgression. Our data provide a better understanding of RGAs, which facilitate genomics-based improvements in disease resistance in sunflower.
向日葵是一种重要的油料作物和全球粮食来源，易受多种病原体影响，这些病原体导致向日葵产量严重损失。利用遗传抗性是预防传染病最经济、有效的措施。基于向日葵泛基因组，在本研究中，我们探索了该物种内抗性基因类似物（RGA）的变异性。根据使用RGAugury管道对向日葵泛基因组中RGA候选物的比较分析，共鉴定出1344个RGA，包括1107个保守RGA、199个变异RGA和38个稀有RGA。我们还在数量性状位点（QTL）鉴定了与向日葵抗（）相关的RGA。共发现61个RGA位于四个数量性状位点（QTL）。通过对一个感病和两个耐病向日葵自交系（IL）在接种后不同时间点的RGA进行详细表达分析，我们发现348个RGA对核盘菌菌核病（SHR）表现出差异表达，其中17个差异表达的RGA位于QTL区域内。此外，有15个RGA候选物存在基因渗入。我们的数据有助于更好地理解RGA，从而促进基于基因组学的向日葵抗病性改良。
