Phytopathology. 2007 May;97(5):574-83. doi: 10.1094/PHYTO-97-5-0574.
ABSTRACT Isolates of Puccinia triticina collected from durum wheat from Argentina, Chile, Ethiopia, France, Mexico, Spain, and the United States were analyzed with 11 simple sequence repeat (SSR) markers in order to determine the genetic relationship among isolates. These isolates also were compared with P. triticina isolates from common wheat from North America, and an isolate collected from Aegilops speltoides from Israel, to determine genetic relationships among groups of P. triticina found on different telial hosts. The large majority of isolates from durum wheat were identical for SSR markers or had <8% genetic dissimilarity, except for isolates from Ethiopia, which had 55% dissimilarity with respect to the other durum isolates. Isolates from common wheat had >70% genetic dissimilarity from isolates from durum wheat, and the isolate from A. speltoides was >90% dissimilar from all isolates tested. Analysis of molecular variance tests showed significant levels (P = 0.001) of genetic differentiation among regions and among isolates within countries. Isolates of P. triticina from durum wheat from South America, North America, and Europe were closely related based on SSR genotypes, suggesting a recent common ancestor, whereas P. triticina from Ethiopia, common wheat, and A. speltoides each had distinct SSR genotypes, which suggested different origins.
摘要 从阿根廷、智利、埃塞俄比亚、法国、墨西哥、西班牙和美国的硬质小麦中采集的小麦柄锈菌分离物，用 11 个简单序列重复（SSR）标记进行分析，以确定分离物之间的遗传关系。这些分离物还与来自北美的普通小麦中的小麦柄锈菌分离物以及来自以色列的节节麦的分离物进行了比较，以确定在不同冬孢子宿主上发现的小麦柄锈菌群体之间的遗传关系。除埃塞俄比亚的分离物与其他硬质小麦分离物的遗传相似性为 55%外，大多数硬质小麦分离物在 SSR 标记上是相同的或遗传差异<8%。普通小麦的分离物与硬质小麦的分离物的遗传相似性>70%，而节节麦的分离物与所有测试的分离物的遗传相似性>90%。分子方差分析测试显示，地区之间和国家内部的遗传分化达到显著水平（P=0.001）。基于 SSR 基因型，来自南美、北美和欧洲的硬质小麦的小麦柄锈菌分离物密切相关，表明有一个最近的共同祖先，而来自埃塞俄比亚、普通小麦和节节麦的小麦柄锈菌分离物每个都有独特的 SSR 基因型，这表明它们有不同的起源。
