Phytopathology. 2003 Jan;93(1):88-95. doi: 10.1094/PHYTO.2003.93.1.88.
ABSTRACT A polymerase chain reaction approach using degenerate primers that targeted the conserved domains of cloned plant disease resistance genes (R genes) was used to isolate a set of 15 resistance gene analogs (RGAs) from common bean (Phaseolus vulgaris). Eight different classes of RGAs were obtained from nucleotide binding site (NBS)-based primers and seven from not previously described Toll/Interleukin-1 receptor-like (TIR)-based primers. Putative amino acid sequences of RGAs were significantly similar to R genes and contained additional conserved motifs. The NBS-type RGAs were classified in two subgroups according to the expected final residue in the kinase-2 motif. Eleven RGAs were mapped at 19 loci on eight linkage groups of the common bean genetic map constructed at Centro Internacional de Agricultura Tropical. Genetic linkage was shown for eight RGAs with partial resistance to anthracnose, angular leaf spot (ALS) and Bean golden yellow mosaic virus (BGYMV). RGA1 and RGA2 were associated with resistance loci to anthracnose and BGYMV and were part of two clusters of R genes previously described. A new major cluster was detected by RGA7 and explained up to 63.9% of resistance to ALS and has a putative contribution to anthracnose resistance. These results show the usefulness of RGAs as candidate genes to detect and eventually isolate numerous R genes in common bean.
摘要 利用针对已克隆植物抗病基因（R 基因）保守结构域的简并引物，通过聚合酶链反应（PCR）方法，从普通菜豆（Phaseolus vulgaris）中分离得到了一组 15 个抗病基因类似物（RGA）。用核苷酸结合位点（NBS）基引物获得了 8 类不同的 RGA，用以前未描述的 Toll/白细胞介素-1 受体样（TIR）基引物获得了 7 类 RGA。RGA 的假定氨基酸序列与 R 基因具有高度相似性，并含有额外的保守基序。NBS 型 RGA 根据激酶-2 基序中预期的最终残基分为两个亚组。在构建的 19 个普通菜豆遗传图谱的 8 个连锁群上的 19 个位点上定位了 11 个 RGA。对 8 个 RGAs 与部分炭疽病、角斑病（ALS）和 Bean golden yellow mosaic virus（BGYMV）的抗性进行了遗传连锁分析。RGA1 和 RGA2 与炭疽病和 BGYMV 的抗性基因座相关，是以前描述的两个 R 基因簇的一部分。RGA7 检测到一个新的主要簇，解释了 ALS 抗性的 63.9%，并可能有助于炭疽病抗性。这些结果表明 RGA 作为候选基因用于检测和最终分离普通菜豆中大量 R 基因的有效性。
