School of Landscape Architecture, Beijing University of Agriculture, Beijing, 102206, China.
Shandong Provincial Center of Forest Tree Germplasm Resources, Shandong Province, Jinan, 250102, China.
Sci Rep. 2021 May 12;11(1):10117. doi: 10.1038/s41598-021-89418-7.
Sorbus pohuashanensis (Hance) Hedl. is a Chinese native alpine tree species, but the problem of introducing S. pohuashanensis to low altitude areas has not been solved. In this study, we aimed to explore the molecular regulatory network of S. pohuashanensis in response to high-temperature stress using RNA-Sequencing technology and physiological and biochemical determination. Based on transcriptomic data, we obtained 1221 genes (752 up-regulated and 469 down-regulated) that were differentially expressed during 8 h 43℃ treatment and candidate genes were related to calcium signaling pathway, plant hormone signal transduction, heat shock factors, chaperones, ubiquitin mediated proteolysis, cell wall modification, ROS scavenging enzymes, detoxification and energy metabolism. The analysis of high temperature response at the physiological level and biochemical level were performed. The chlorophyll fluorescence parameters of leaf cells decreased, the content of osmotic regulators increased, and the activity of ROS scavenging enzymes decreased. The molecular regulatory network of S. pohuashanensis in response to high-temperature stress was preliminarily revealed in this study, which provides fundamental information improving introducing methods and discovering heat-tolerant genes involved in high-temperature stress in this species and provides a reference for other plants of the genus Sorbus.
华西稠李（Sorbus pohuashanensis (Hance) Hedl.）是中国特有的高山树种，但将其引入低海拔地区的问题尚未得到解决。本研究旨在利用 RNA 测序技术和生理生化测定，探索华西稠李响应高温胁迫的分子调控网络。基于转录组数据，我们获得了 1221 个差异表达基因（752 个上调和 469 个下调），这些基因在 43℃处理 8 h 时表达差异显著，候选基因与钙信号通路、植物激素信号转导、热休克因子、伴侣蛋白、泛素介导的蛋白水解、细胞壁修饰、ROS 清除酶、解毒和能量代谢有关。我们还对高温胁迫的生理和生化水平进行了分析。结果表明，叶片细胞的叶绿素荧光参数下降，渗透调节剂的含量增加，ROS 清除酶的活性降低。本研究初步揭示了华西稠李响应高温胁迫的分子调控网络，为该物种的引种方法改进和发现与高温胁迫相关的耐热基因提供了基础信息，也为其他稠李属植物提供了参考。
