Phytopathology. 2003 Apr;93(4):436-42. doi: 10.1094/PHYTO.2003.93.4.436.
ABSTRACT Viability of the postharvest biocontrol agent Candida sake CPA-1 stored as liquid formulation was evaluated by studying the effect of growth, preservation medium, and temperature. C. sake was grown in molasses medium with unmodified water activity (a(w)) and in the same with a(w) modified to 0.98 with the addition of several solutes. Cells were preserved with isotonic solutions of different substances. Efficacy of liquid formulations stored for different periods was tested against infection by Penicillium expansum on apples. The best growth media were the (unmodified one and those modified to 0.98 a(w) with the addition of glycerol or sorbitol. For all growth media, the best preservation medium was the isotonic solution prepared with trehalose. When the effect of trehalose concentration in the preservation medium was studied, generally, at trehalose concentrations below the isotonic one, C. sake viabilities increased with increased trehalose. However, the best results were obtained when cells were preserved with the trehalose solution which was isotonic with cells. After 7 months of storage at 4 degrees C, cells that were grown in the sorbitol-modified medium and preserved with the isotonic solution of trehalose (0.96 M) maintained their viability and efficacy against P. expansum infection of apples.
摘要 通过研究生长、保存介质和温度对保鲜剂液体制剂中后生生物防治剂假丝酵母（Candida sake）CPA-1 的影响，评估了其储存后的活性。假丝酵母在未修改水活度（a(w)）的糖蜜培养基和添加几种溶质以将 a(w)修改为 0.98 的相同培养基中生长。细胞用不同物质的等渗溶液保存。测试了不同保存期的液体制剂对苹果扩展青霉感染的防治效果。最佳生长培养基为（未修改的培养基和用甘油或山梨糖醇修改为 0.98 a(w)的培养基）。对于所有生长培养基，最佳保存培养基是用海藻糖制备的等渗溶液。当研究保存培养基中海藻糖浓度的影响时，通常在低于等渗浓度的海藻糖浓度下，假丝酵母的活力随海藻糖浓度的增加而增加。然而，当用与细胞等渗的海藻糖溶液保存细胞时，可获得最佳结果。在 4°C 下储存 7 个月后，在山梨糖醇修饰培养基中生长并保存在海藻糖等渗溶液（0.96 M）中的细胞保持其活力和防治苹果扩展青霉感染的功效。
