Phytopathology. 1998 Sep;88(9):960-4. doi: 10.1094/PHYTO.1998.88.9.960.
ABSTRACT Unmodified and low water activity (a(w))-tolerant cells of Candida sake CPA-1 applied before harvest were compared for ability to control blue mold of apples ('Golden Delicious') caused by Penicillium expansum under commercial storage conditions. The population dynamics of strain CPA-1 on apples were studied in the orchard and during storage following application of 3 x 10(6) CFU/ml of each treatment 2 days prior to harvest. In the field, the population size of the unmodified treatment remained relatively unchanged, while the population size of the low-a(w)-modified CPA-1 cells increased. During cold storage, the populations in both treatments increased from 10(3) to 10(5) CFU/g of apple after 30 days, and then declined to about 2.5 x 10(4) CFU/g of apple. In laboratory studies, the low-a(w)-tolerant cells provided significantly better disease control as compared with the unmodified cells and reduced the number of infected wounds and lesion size by 75 and 90%, respectively, as compared with the non-treated controls. After 4 months in cold storage, both unmodified and low-a(w)-tolerant cells of C. sake were equally effective against P. expansum on apple (>50% reduction in size of infected wounds).
摘要 在商业贮藏条件下，比较了收获前应用未经修饰和低水活度（a(w)）耐受细胞的清酒假丝酵母 CPA-1 对青霉扩展引起的苹果（“金冠”）青霉病的控制能力。在果园和贮藏期间研究了 3x10(6)cfu/ml 各处理在收获前 2 天应用于苹果后，CPA-1 菌株在苹果上的种群动态。在田间，未经修饰处理的种群大小相对保持不变，而低 a(w)修饰的 CPA-1 细胞的种群大小增加。在冷藏过程中，在 30 天后，两种处理的种群均从 10(3)增加到 10(5)cfu/g 苹果，然后下降到约 2.5x10(4)cfu/g 苹果。在实验室研究中，与未经修饰的细胞相比，低 a(w)耐受细胞提供了显著更好的疾病控制，并将感染伤口的数量和病变大小分别减少了 75%和 90%，与未处理的对照相比。在冷藏 4 个月后，未经修饰和低 a(w)耐受的清酒假丝酵母细胞对苹果上的扩展青霉（感染伤口大小减少 50%以上）同样有效。
