Phytopathology. 2004 Nov;94(11):1164-71. doi: 10.1094/PHYTO.2004.94.11.1164.
ABSTRACT Cranberry stem gall is characterized by tumors that girdle stems, thereby killing all distal leaves, flowers, and fruit. Among bacteria isolated from galls, all 11 isolates that were identified as members of the family Enterobacteriaceae caused galls on 50 to 100% of micropropagated cranberry plants that were inoculated. Four of fifteen isolates identified as Pseudomonas spp. caused galls on 10 to 83% of plants inoculated. Twelve of fifteen isolates identified as either Agrobacterium spp. or Rhizobium spp. caused galls on 10 to 50% of plants inoculated, but the galls were smaller than those caused by members of the family Enterobacteriaceae or Pseudomonas spp. There was a positive correlation between the ability of bacteria to produce IAA in vitro and cause galls. In 2002 and 2003, bacteria were isolated from plant and soil samples collected from beds where stem gall had been observed in the past 2 years and beds where stem gall had never been observed. IAA-producing bacteria were common in all samples, although trends were different across years. The results of this study support the hypothesis that IAA-producing bacteria cause cranberry stem gall and suggest that rather than one bacterial species being the cause, multiple strains of bacteria that produce IAA may be responsible for gall formation.
蔓越莓茎瘤的特征是肿瘤环绕茎干，从而杀死所有远端的叶片、花朵和果实。从肿瘤中分离出的 11 株细菌中，有 11 株被鉴定为肠杆菌科的成员，它们会导致 50%到 100%接种的微繁殖越橘植物产生肿瘤。15 株被鉴定为假单胞菌的菌株中有 4 株会导致 10%到 83%接种的植物产生肿瘤。15 株被鉴定为根瘤菌或根癌土壤杆菌的菌株中有 12 株会导致 10%到 50%接种的植物产生肿瘤，但肿瘤比肠杆菌科或假单胞菌成员引起的肿瘤小。细菌在体外产生 IAA 的能力与引起肿瘤的能力之间存在正相关。在 2002 年和 2003 年，从过去 2 年中观察到茎瘤的床和从未观察到茎瘤的床中收集的植物和土壤样本中分离出了细菌。尽管各年的趋势不同，但所有样本中都存在产生 IAA 的细菌。本研究的结果支持了产生 IAA 的细菌引起越橘茎瘤的假说，并表明引起肿瘤形成的原因不是一种细菌，而是多种产生 IAA 的细菌菌株。
