Khianngam Saowapar, Meetum Pimjai, Chiangmai Pantipa Na, Tanasupawat Somboon
Faculty of Animal Sciences and Agricultural Technology, Silpakorn University, Phetchaburi Information Technology Campus, Phetchaburi 76120, Thailand.
Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
Trop Life Sci Res. 2023 Mar;34(1):219-239. doi: 10.21315/tlsr2023.34.1.12. Epub 2023 Mar 31.
Indole-3-acetic acid (IAA) is one of the most physiologically active auxins produced by rhizobacteria and is potentially applied for agriculture. Two endophytic bacteria, VR2 and MG9, isolated from the root of (L.) collected at Cha-Am, and the leaf of (L.) Blume collected from a mangrove forest at Ban Laem, Phetchaburi Province, Thailand, were taxonomic characterised based on their phenotypic characteristics and 16S rRNA gene analysis. Strain VR2 was closely related to CIP 103441 (99.6% similarity), while strain MG9 was closely related to B8W22 (99.9% similarity). Consequently, they were identified as and , respectively. The IAA production of VR2 and MG9 strains are determined and applied to rice seeds for their root and shoot germination. Strains VR2 and MG9 greatly produced a yield of IAA, 246.00 and 195.55 μg/mL in 1,000 μg/mL of L-tryptophan at pH 6 for 48 h. They showed no significant differences in IAA to root and shoot development. However, the bacterial IAA exhibited potential nearby synthetic IAA, which had a significant effect compared to the control. IAA produced from these two strains might preferably trim down the use of synthetic IAA and could contribute to sustainable agriculture.
吲哚 - 3 - 乙酸(IAA)是根际细菌产生的生理活性最强的生长素之一,具有潜在的农业应用价值。从泰国巴蜀府班莱姆红树林采集的(L.)叶片以及差安采集的(L.)根部分离出的两株内生细菌VR2和MG9,基于其表型特征和16S rRNA基因分析进行了分类鉴定。菌株VR2与CIP 103441密切相关(相似度99.6%),而菌株MG9与B8W22密切相关(相似度99.9%)。因此,它们分别被鉴定为和。测定了VR2和MG9菌株的IAA产量,并将其应用于水稻种子的根和芽萌发。在pH 6、1000μg/mL L - 色氨酸条件下培养48小时,VR2和MG9菌株大量产生IAA,产量分别为246.00和195.55μg/mL。它们对根和芽发育的IAA作用无显著差异。然而,细菌IAA表现出与合成IAA相近的潜力,与对照相比有显著效果。这两株菌株产生的IAA可能有助于减少合成IAA的使用,并为可持续农业做出贡献。