Phytopathology. 1999 Apr;89(4):281-5. doi: 10.1094/PHYTO.1999.89.4.281.
ABSTRACT Three F(2) populations derived from crosses between the resistant cultivar AB 136 and the susceptible cultivar Michelite (MiA), and one F(2) population derived from a cross between AB 136 and Mexico 222 (MeA), were used to identify markers linked to anthracnose resistance genes present in cultivar AB 136. Primer OPZ04 produced a DNA band (OPZ04(560)) linked in coupling phase to the resistance gene for pathotype 89 (8.5 +/- 0.025 cM) in one population derived from the cross MiA. In the same population, primer OPZ09 produced one band (OPZ09(950)) linked in repulsion phase (20.4 +/- 0.014 cM) to the same resistance gene. The simultaneous use of markers in coupling and in repulsion phases allowed the identification of the three genotypic classes. In the other two populations from cross MiA, OPZ04(560) was linked in coupling phase to resistance genes for pathotypes 73 (2.9 +/- 0.012 cM) and 81 (2.8 +/- 0.017 cM). In population MeA, OPZ04(560) was linked in coupling phase (7.5 +/- 0.033 cM) to resistance to pathotype 64. These data suggest that a single gene or complex locus of linked resistance genes present in cultivar AB 136 confers resistance to all four pathotypes of C. lindemuthianum.
摘要 利用 AB 136 与 Michelite (MiA) 杂交得到的三个 F2 群体和 AB 136 与 Mexico 222 (MeA) 杂交得到的一个 F2 群体，鉴定与 AB 136 中存在的炭疽病抗性基因连锁的标记。引物 OPZ04 在来源于 MiA 的一个群体中产生一个与路径型 89 的抗性基因连锁的 DNA 带（OPZ04(560)），位于偶合相中（8.5 +/- 0.025 cM）。在同一群体中，引物 OPZ09 产生一个与同一抗性基因连锁的排斥相的带（OPZ09(950)）（20.4 +/- 0.014 cM）。在偶合相和排斥相同时使用标记可以鉴定出三种基因型。在来源于 MiA 的另外两个群体中，OPZ04(560)与路径型 73（2.9 +/- 0.012 cM）和 81（2.8 +/- 0.017 cM）的抗性基因连锁在偶合相中。在 MeA 群体中，OPZ04(560)与路径型 64 的抗性基因连锁在偶合相中（7.5 +/- 0.033 cM）。这些数据表明，AB 136 中存在的单个基因或连锁抗性基因的复杂基因座赋予其对所有四种 C. lindemuthianum 路径型的抗性。
