Phytopathology. 1999 Aug;89(8):695-700. doi: 10.1094/PHYTO.1999.89.8.695.
ABSTRACT We devised a rapid technique for the objective and precise assessment of both the pathogenicity of maize streak virus (MSV) isolates and the MSV resistance of maize genotypes. The technique involves the use of agroinoculation to infect maize seedlings and the objective symptom evaluation by quantification of infection rates, stunting, and chlorotic leaf areas. In assessing the MSV resistance of 19 maize genotypes, we describe how the use of differentially virulent virus isolates enables the analysis of MSV resistance phenotypes, ranging from extremely susceptible to completely immune. We further demonstrate how quantification of chlorotic leaf areas by image analysis permits differentiation between degrees of MSV resistance that are indistinguishable from one another using currently employed symptom assessment approaches. Using chlorotic area measurements, we quantify the virulence of a diverse group of 10 MSV isolates and, through agroinoculation of differentially susceptible maize genotypes, we demonstrate the use of our technique in evaluating the pathogenicity of these isolates.
摘要 我们设计了一种快速技术，用于客观、准确地评估玉米条纹病毒 (MSV) 分离株的致病性和玉米基因型对 MSV 的抗性。该技术涉及使用农杆菌接种感染玉米幼苗，并通过量化感染率、矮化和黄化叶片面积来进行客观症状评估。在评估 19 个玉米基因型对 MSV 的抗性时，我们描述了如何使用毒力不同的病毒分离株来分析 MSV 抗性表型，范围从极敏感到完全免疫。我们进一步展示了如何通过图像分析量化黄化叶片面积，从而区分目前采用的症状评估方法无法区分的 MSV 抗性程度。使用黄化面积测量值，我们量化了一组 10 种 MSV 分离株的毒力，并通过接种对 MSV 敏感性不同的玉米基因型，证明了我们的技术在评估这些分离株的致病性方面的应用。
