Phytopathology. 1999 Mar;89(3):247-53. doi: 10.1094/PHYTO.1999.89.3.247.
ABSTRACT Sporisorium reilianum f. sp. zeae is the causal agent of maize head smut, a disease present in several regions of France. A cytological study was carried out to describe a key step of the fungal etiology, in which the mycelium invades the vegetative shoot apex. Light and transmission electron microscopy observations show that the fungus is mostly intracellular and suggest that it passes through the host cell wall by lysis and mechanical pressure. The hyphae are surrounded by an amorphous vesicle-rich layer limited by a membrane related to the host plasmalemma. The encasement can be considered as an exchange zone between the plant and the fungus. The infected host cells appear normal; therefore, the fungus seems to act like a biotrophic endophyte.
玉米丝黑穗病菌（Sporisorium reilianum f. sp. zeae）是引起玉米丝黑穗病的病原菌，该病害在法国的多个地区均有发生。本研究对该病原菌的致病过程进行了细胞学研究，特别是对其菌丝侵染营养芽顶端这一关键步骤进行了研究。通过光镜和电镜观察发现，该病原菌主要以胞内寄生的方式存在，其穿过寄主细胞壁的方式可能与溶菌和机械压力有关。该病原菌的菌丝被一层富含囊泡的无定形层所包围，这层囊泡层被一层与寄主质膜相关的膜所限制。这种被膜可以被认为是植物和真菌之间的交换区。受感染的寄主细胞看起来正常，因此，该真菌似乎像一种生物营养型内生真菌。
