Phytopathology. 1998 Dec;88(12):1283-93. doi: 10.1094/PHYTO.1998.88.12.1283.
ABSTRACT Genetic variation within a worldwide collection of 208 isolates of Fu-sarium oxysporum f. sp. cubense, representing physiological races 1, 2, 3, and 4 and the 20 reported vegetative compatibility groups (VCGs), was analyzed using modified DNA amplification fingerprinting. Also characterized were 133 isolates that did not belong to any of the reported VCGs of F. oxysporum f. sp. cubense including race 3 isolates from a Heliconia species and isolates from a symptomatic wild banana species growing in the jungle in peninsular Malaysia. The DNA fingerprint patterns were generally VCG specific, irrespective of geographic or host origin. A total of 33 different genotypes were identified within F. oxysporum f. sp. cu-bense; 19 genotypes were distinguished among the isolates that belonged to the 20 reported VCGs, and 14 new genotypes were identified among the isolates that did not belong to any of the existing VCGs. DNA fingerprinting analysis also allowed differentiation of nine clonal lineages within F. oxysporum f. sp. cubense. Five of these lineages each contained numerous closely related VCGs and genotypes, and the remaining four lineages each contained a single genotype. The genetic diversity and geographic distribution of several of these lineages of F. oxysporum f. sp. cubense suggests that they have coevolved with edible bananas and their wild diploid progenitors in Asia. DNA fingerprinting analysis of isolates from the wild pathosystem provides further evidence for the coevolution hypothesis. The genetic isolation and limited geographic distribution of four of the lineages of F. oxysporum f. sp. cubense suggests that the pathogen has also arisen independently, both within and outside of the center of origin of the host.
摘要 使用改良 DNA 扩增指纹分析对来自全球 208 株尖孢镰刀菌古巴专化型（Fusarium oxysporum f. sp. cubense）的遗传变异进行了分析，这些菌株代表生理小种 1、2、3 和 4 以及 20 个报道的营养体亲和群（vegetative compatibility groups，VCGs）。还对 133 个不属于任何报道的 F. oxysporum f. sp. cubense VCG 的菌株进行了特征描述，其中包括来自 Heliconia 物种的 3 个小种和在马来西亚半岛丛林中生长的有症状野生香蕉物种的菌株。DNA 指纹图谱通常与 VCG 特异性有关，无论地理或宿主起源如何。在尖孢镰刀菌古巴专化型中总共鉴定出 33 种不同的基因型；在属于 20 个报道的 VCG 的菌株中区分出 19 种基因型，在不属于任何现有 VCG 的菌株中鉴定出 14 种新基因型。DNA 指纹分析还允许在尖孢镰刀菌古巴专化型内区分出 9 个克隆谱系。这些谱系中的 5 个谱系每个都包含许多密切相关的 VCG 和基因型，而其余 4 个谱系每个都包含一个单一的基因型。这些尖孢镰刀菌古巴专化型的几个谱系的遗传多样性和地理分布表明，它们与可食用香蕉及其在亚洲的野生二倍体祖先共同进化。对野生病理系统中分离物的 DNA 指纹分析为共同进化假说提供了进一步的证据。四个尖孢镰刀菌古巴专化型谱系的遗传隔离和有限的地理分布表明，病原体也在宿主起源中心内外独立出现。
