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根据基因组数据设计的特异性PCR引物，用于快速检测香牙蕉中尖孢镰刀菌古巴专化型热带4号生理小种。

Specific PCR primer designed from genome data for rapid detection of Fusarium oxysporum f. sp. cubense tropical race 4 in the Cavendish banana.

作者信息

Nozawa Shunsuke, Pangilinan Dan Charlie Joy, Dionisio G Alvindia, Watanabe Kyoko

机构信息

School of Agriculture, Tamagawa University, Machida, Tokyo, Japan.

Philippine Center for Postharvest Development and Mechanization, Nueva Ecija, Philippines.

出版信息

PLoS One. 2024 Dec 2;19(12):e0313358. doi: 10.1371/journal.pone.0313358. eCollection 2024.

DOI:10.1371/journal.pone.0313358
PMID:39621667
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11611109/
Abstract

Fusarium wilt caused by Fusarium oxysporum f. sp. cubense (Foc) tropical race 4 (TR4) severely affects banana production worldwide. Thus, specific PCR primers have been developed to rapidly diagnose and monitor Foc TR4-related fusarium wilt outbreaks in bananas. However, evaluation of these primers revealed room for improvement in the accuracy. This study aimed to design highly specific PCR primers based on genome data for Foc TR4 downloaded from the National Center for Biotechnology Information database. The specificity of the primers was assessed using Foc TR4, Foc races 1 and 2, and 15 other formae speciales strains. The utility of the primers was verified by correctly detecting Foc TR4 in 7 out of 86 isolates of Fusarium spp. obtained from banana farms in the Philippines. The primers allowed for rapid detection in experimentally diseased tissues. We concluded that this novel primer set enables the simplified diagnosis of fusarium wilt caused by Foc TR4 in bananas.

摘要

由尖孢镰刀菌古巴专化型热带4号小种（Foc TR4）引起的香蕉枯萎病严重影响全球香蕉生产。因此，已开发出特异性PCR引物，用于快速诊断和监测香蕉中与Foc TR4相关的枯萎病爆发。然而，对这些引物的评估显示在准确性方面仍有改进空间。本研究旨在基于从美国国立生物技术信息中心数据库下载的Foc TR4基因组数据设计高度特异性的PCR引物。使用Foc TR4、Foc 1号和2号小种以及其他15个专化型菌株评估引物的特异性。通过正确检测从菲律宾香蕉种植园获得的86株镰刀菌属分离物中的7株Foc TR4，验证了引物的实用性。这些引物可在实验性患病组织中进行快速检测。我们得出结论，这套新型引物能够简化对香蕉中由Foc TR4引起的枯萎病的诊断。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6099/11611109/349c424d589f/pone.0313358.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6099/11611109/5cef919c4fad/pone.0313358.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6099/11611109/a32ba49a7b54/pone.0313358.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6099/11611109/065e123798a4/pone.0313358.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6099/11611109/88d329d578fa/pone.0313358.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6099/11611109/349c424d589f/pone.0313358.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6099/11611109/5cef919c4fad/pone.0313358.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6099/11611109/a32ba49a7b54/pone.0313358.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6099/11611109/065e123798a4/pone.0313358.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6099/11611109/88d329d578fa/pone.0313358.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6099/11611109/349c424d589f/pone.0313358.g005.jpg

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