Phytopathology. 1998 Dec;88(12):1351-8. doi: 10.1094/PHYTO.1998.88.12.1351.
ABSTRACT Spiroplasma citri, transmitted by phloem-feeding leafhoppers, moves from the gut lumen through the gut wall, hemolymph, and salivary glands and multiplies in insect tissues. Nontransmissible lines were deficient in their ability to cross these barriers. Molecular analysis revealed extensive chromosomal rearrangements between the transmissible and nontransmissible spiroplasma lines including a large chromosomal inversion and deletions of about 10 kb at each inversion border. One open reading frame of the deleted region, cloned from the transmissible strain BR3-3X, encodes an integral membrane protein of 58 kDa that shares limited sequence similarity with major adhesin proteins of two zoopathogenic mycoplasmas. Adhesion of spiroplasmas to cultured leafhopper cells was inhibited by proteases, suggesting that adherence to host cells is mediated by spiroplasma membrane protein(s). A hypothetical model for insect transmission of phytopathogenic mollicutes is presented.
摘要 由韧皮部取食的叶蝉传播的柑橘衰退螺旋体通过肠腔、肠壁、血淋巴和唾液腺移动，并在昆虫组织中繁殖。不可传播的品系在穿过这些屏障的能力上存在缺陷。分子分析显示，可传播和不可传播的螺旋体品系之间存在广泛的染色体重排，包括一个大的染色体倒位和每个倒位边界处约 10 kb 的缺失。从可传播菌株 BR3-3X 中克隆的缺失区域的一个开放阅读框，编码一个 58 kDa 的完整膜蛋白，与两种动物病原支原体的主要粘附蛋白有有限的序列相似性。蛋白酶抑制了螺旋体对培养的叶蝉细胞的粘附，表明对宿主细胞的粘附是由螺旋体膜蛋白介导的。提出了一种植物病原柔膜体昆虫传播的假设模型。
