Shimooka Makoto, Sakurai Yoshimi, Muramatsu Yasukazu, Uchida Leo
School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu 069-8501, Hokkaido, Japan.
Insects. 2021 Nov 25;12(12):1056. doi: 10.3390/insects12121056.
Species of the genus are common within arthropods and plants worldwide. Mosquito-associated spp. have been reported to show pathogenicity toward mosquitoes, which serve as vectors of several infectious diseases that have detrimental effects on public health. Although spp. are expected to have potential use as biological vector-control tools, characteristics such as their distribution, host species, and cytopathogenic effects (CPEs) are not well understood. In this study, we isolated a sp. from a female collected in Hokkaido, northern Japan. Phylogenetic analysis based on the 16S rRNA gene sequence indicated our isolate was closely related to . We screened 103 mosquito pools consisting of 3 genera and 9 species, but only detected in the first isolation. In an in vitro assay, our isolate grew well at 28 °C, but no propagation was observed at 37 °C. Furthermore, the isolate showed strong CPE on a mosquito-derived cultured cell line (C6/36), and its propagation slightly increased when co-cultured with C6/36 cells. To our knowledge, this is the third report of the isolation of from mosquitoes and the first case in Asia. Our findings provide epidemiological data on distribution in the region.
该属的物种在全球节肢动物和植物中很常见。据报道，与蚊子相关的该属物种对蚊子具有致病性，而蚊子是几种对公众健康有不利影响的传染病的传播媒介。尽管该属物种有望作为生物媒介控制工具具有潜在用途，但其分布、宿主物种和细胞致病效应（CPEs）等特征尚未得到充分了解。在本研究中，我们从日本北部北海道采集的一只雌性蚊子中分离出一种该属物种。基于16S rRNA基因序列的系统发育分析表明，我们分离出的菌株与该属的某一物种密切相关。我们筛选了由3个属和9个物种组成的103个蚊子样本池，但仅在首次分离中检测到该属物种。在体外试验中，我们分离出的菌株在28℃下生长良好，但在37℃下未观察到繁殖。此外，该分离株在蚊子来源的培养细胞系（C6/36）上表现出强烈的CPE，并且当与C6/36细胞共培养时其繁殖略有增加。据我们所知，这是从蚊子中分离出该属物种的第三份报告，也是亚洲的首例。我们的研究结果提供了该属物种在该地区分布的流行病学数据。
