Phytopathology. 1998 Jul;88(7):648-57. doi: 10.1094/PHYTO.1998.88.7.648.
ABSTRACT The biological and molecular properties of Sinaloa tomato leaf curl virus (STLCV) were investigated in line with the hypothesis that STLCV is a previously uncharacterized, whitefly-transmitted geminivirus from North America. STLCV causes yellow leaf curl symptoms in tomato and yellow-green foliar mottle in pepper. Five species belonging to two plant families were STLCV experimental hosts. STLCV had a persistent relationship with its whitefly vector, Bemisia tabaci. Polymerase chain reaction fragments of STLCV common region (CR) sequences of the A or B genomic components and the viral coat protein gene (AV1) were molecularly cloned and sequenced. The STLCV A- and B-component CR sequences (174 nucleotides each) shared 97.9% identity and contained identical cis elements putatively involved in transcriptional regulation and an origin of replication (the AC cleavage site within the loop of the hairpin structure and two direct repeat sequences thought to constitute the Rep binding motif), which collectively are diagnostic for subgroup III geminiviruses. The STLCV CR sequence shared 23.1 to 77.6% identity with CR sequences of representative geminiviridae, indicating the STLCV CR sequence is unique. Molecular phylogenetic analysis of CR or AV1 sequences of STLCV and the respective sequences of 31 familial members supported the placement of STLCV as a unique bipartite, subgroup III virus most closely related to other viruses from the Western Hemisphere. STLCV is provisionally described as a new species within the genus Begomovirus, family Geminiviridae.
摘要 本研究旨在探究 Sinaloa 番茄曲叶病毒（STLCV）的生物学和分子特性，从而验证该病毒是一种尚未被鉴定的、由北美的粉虱传播的双生病毒的假说。STLCV 可引起番茄黄卷叶病和辣椒黄-绿斑驳病。有 5 个科的 5 个种被鉴定为 STLCV 的实验宿主。STLCV 与其粉虱介体 Bemisiatabaci 存在持久的共生关系。STLCV 的 A 或 B 基因组成分的共同区（CR）序列和病毒外壳蛋白基因（AV1）的聚合酶链反应片段被克隆和测序。STLCV 的 A 和 B 组分 CR 序列（各 174 个核苷酸）的同源性为 97.9%，包含相同的顺式作用元件，推测这些元件参与转录调控和复制起点（发夹结构环内的 AC 切割位点和两个直接重复序列，认为它们构成 Rep 结合基序），这些元件是亚组 III 双生病毒的特征。STLCV 的 CR 序列与代表双生病毒科的 CR 序列的同源性为 23.1%至 77.6%，表明 STLCV 的 CR 序列是独特的。对 STLCV 的 CR 或 AV1 序列和 31 个家族成员的相应序列的分子系统发育分析支持将 STLCV 作为一个独特的二分体、亚组 III 病毒进行归类，该病毒与西半球的其他病毒关系最为密切。STLCV 被暂时描述为双生病毒属中的一个新种，属于双生病毒科。
