Phytopathology. 1998 Jul;88(7):678-84. doi: 10.1094/PHYTO.1998.88.7.678.
ABSTRACT Application of salicylic acid induces systemic acquired resistance in tobacco. pchA and pchB, which encode for the biosynthesis of salicylic acid in Pseudomonas aeruginosa, were cloned into two expression vectors, and these constructs were introduced into two root-colonizing strains of P. fluorescens. Introduction of pchBA into strain P3, which does not produce salicylic acid, rendered this strain capable of salicylic acid production in vitro and significantly improved its ability to induce systemic resistance in tobacco against tobacco necrosis virus. Strain CHA0 is a well-described biocontrol agent that naturally produces salicylic acid under conditions of iron limitation. Introduction of pchBA into CHA0 increased the production of salicylic acid in vitro and in the rhizosphere of tobacco, but did not improve the ability of CHA0 to induce systemic resistance in tobacco. In addition, these genes did not improve significantly the capacity of strains P3 and CHA0 to suppress black root rot of tobacco in a gnotobiotic system.
摘要 水杨酸的应用可诱导烟草获得系统抗性。铜绿假单胞菌中编码水杨酸生物合成的 pchA 和 pchB 被克隆到两个表达载体中，并将这些构建体引入到两个根定殖型荧光假单胞菌菌株中。将 pchBA 导入不产生水杨酸的菌株 P3 中，可使该菌株在体外产生水杨酸，并显著提高其诱导烟草对烟草坏死病毒系统抗性的能力。菌株 CHA0 是一种经过充分描述的生物防治剂，在缺铁条件下可自然产生水杨酸。将 pchBA 导入 CHA0 可增加其在体外和烟草根际中水杨酸的产生，但并未提高 CHA0 诱导烟草系统抗性的能力。此外，这些基因并未显著提高菌株 P3 和 CHA0 在无菌体系中抑制烟草黑胫病的能力。
