Eraslan Gokhan, Kanbur Murat, Silici Sibel, Altinordulu Sule, Karabacak Mursel
Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Erciyes, Kayseri, Turkey.
Exp Anim. 2008 Oct;57(5):453-60. doi: 10.1538/expanim.57.453.
Twenty eight female Wistar rats weighing 150-200 g were used in this study and these animals were divided into 4 groups, each comprising 7 rats. The first group served as the control group, and groups 2, 3, and 4 were administered a single dose of 250 mg/kg.bw propolis, a single dose of 125 mg/kg.bw (1/2LD(50)) cypermethrin, and a single dose of 125 mg/kg.bw cypermethrin followed by a single dose of 250 mg/kg.bw propolis 30 min later, per os using a catheter, respectively. Twenty-four hours after propolis administration, blood and tissue (liver, kidney, and brain) samples were collected. Serum glucose, triglyceride, uric acid, cholesterol, aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) activities/levels, plasma and tissue malondialdehyde (MDA) levels, and erythrocyte and tissue superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activities were determined. Compared to group 1, significant increases in plasma and tissue MDA levels and kidney GSH-Px activity, and significant decreases in erythrocyte SOD and CAT, liver SOD and GSH-Px, kidney SOD and brain SOD, CAT and GSH-Px activities were determined in group 3. Compared to group 1, a significant increase in glucose and a significant decrease in triglyceride levels were determined in group 3. Values pertaining to group 4 were demonstrated to be closer to those of group 1.
本研究使用了28只体重为150 - 200克的雌性Wistar大鼠，这些动物被分为4组，每组7只。第一组作为对照组，第2、3、4组分别经导管口服给予单剂量250毫克/千克体重的蜂胶、单剂量125毫克/千克体重（1/2LD(50)）的氯氰菊酯，以及单剂量125毫克/千克体重的氯氰菊酯，30分钟后再给予单剂量250毫克/千克体重的蜂胶。给予蜂胶24小时后，采集血液和组织（肝脏、肾脏和大脑）样本。测定血清葡萄糖、甘油三酯、尿酸、胆固醇、天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）和碱性磷酸酶（ALP）的活性/水平、血浆和组织丙二醛（MDA）水平，以及红细胞和组织超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px）的活性。与第1组相比，第3组血浆和组织MDA水平以及肾脏GSH-Px活性显著升高，红细胞SOD和CAT、肝脏SOD和GSH-Px、肾脏SOD和大脑SOD、CAT和GSH-Px活性显著降低。与第1组相比，第3组葡萄糖显著升高，甘油三酯水平显著降低。第4组的值显示更接近第1组。
