Singla Shivani, Kumar Neelima R, Kaur Jaspreet
Department of Zoology, Panjab University, Chandigarh, India.
Deparment of Biotechnology, University Institute of Engineering and Technology, Panjab University, Chandigarh, India.
Toxicol Int. 2014 May;21(2):191-5. doi: 10.4103/0971-6580.139808.
Since anticancer drugs are to be administered for long durations of time and are associated with systemic toxicities, the present studies were conducted to evaluate the protective potential of honey bee propolis against a widely used anticancer drug, doxorubicin (DXR) induced toxicity and oxidative damage in liver tissues of rats.
Sixteen male Sprague Dawley rats, weighing between 200-220 g, were used and were divided into four equal groups. Propolis was given orally to rats [250 mg/kg body weight (bw) for 14 consecutive days] and DXR [25 mg/kg bw; intraperitoneally (i.p) was administered on 12(th), 13(th) and 14(th) day of the experiment. All the animals were sacrificed on day 15(th) day by decapitation. Blood and tissue samples were collected for measurement of toxicity and oxidative damage parameters (enzymatic assays and biochemical estimations).
Administration of DXR for 3 days at a cumulative dose of 25 mg/kg bw, induced toxicity and oxidative stress in rats as significantly decreased activity of catalase (CAT), superoxide dismutase (SOD), glutathione-S-transferase (GST), glutathione peroxidase (GSH-Px) and glutathione reductase (GR) were observed in rat liver supernatants when compared to control group. Increased activity of serum glutamic pyruvic transaminase (SGPT) and serum glutamic oxaloacetic transaminase (SGOT) was obtained in DXR administered rats. Also there are significantly increased levels of lipid peroxides (measured as malondialdehyde formation) and significantly decreased level of glutathione (GSH) in doxorubicin treated rat liver supernatants as compared to healthy controls. On the other hand, administration of animals with propolis prior to DXR treatment led to significant modulation of the oxidative damage related parameters in liver and hepatotoxicity parameters in blood, when compared to doxorubicin treated group. However results were still not comparable to control group or only propolis group indicating partial protection by propolis at the concentration used against anticancer drug toxicity.
Propolis extract was found to have a protective effect against doxorubicin-induced toxicity in rat liver though it was still not normalized. It can be concluded that propolis provides partial protection from toxicity of anticancer drug.
由于抗癌药物需长期给药且伴有全身毒性，因此开展了本研究，以评估蜜蜂蜂胶对一种广泛使用的抗癌药物阿霉素（DXR）诱导的大鼠肝脏组织毒性和氧化损伤的潜在保护作用。
选用16只体重在200 - 220克之间的雄性Sprague Dawley大鼠，将其分为四组，每组数量相等。给大鼠口服蜂胶[250毫克/千克体重（bw），连续14天]，并在实验的第12、13和14天腹腔注射（i.p.）DXR[25毫克/千克体重]。在第15天通过断头处死所有动物。采集血液和组织样本，用于测量毒性和氧化损伤参数（酶促测定和生化评估）。
以25毫克/千克体重的累积剂量连续3天给予DXR，可诱导大鼠产生毒性和氧化应激，与对照组相比，大鼠肝脏上清液中过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、谷胱甘肽 - S - 转移酶（GST）、谷胱甘肽过氧化物酶（GSH - Px）和谷胱甘肽还原酶（GR）的活性显著降低。给予DXR的大鼠血清谷丙转氨酶（SGPT）和血清谷草转氨酶（SGOT）活性升高。与健康对照组相比，阿霉素处理的大鼠肝脏上清液中脂质过氧化物水平（以丙二醛形成量衡量）显著升高，谷胱甘肽（GSH）水平显著降低。另一方面，与阿霉素处理组相比，在DXR处理前给动物服用蜂胶可显著调节肝脏中与氧化损伤相关的参数以及血液中的肝毒性参数。然而，结果仍与对照组或仅服用蜂胶组不可比，表明在所使用的浓度下，蜂胶对抗癌药物毒性具有部分保护作用。
发现蜂胶提取物对阿霉素诱导的大鼠肝脏毒性具有保护作用，尽管尚未使其恢复正常。可以得出结论，蜂胶可对抗癌药物毒性提供部分保护。
